I have searched for the methods where I do not require any cell or cell line, but I could not find it. I want to check the anticancer property of the given compound (protein) without using cells. Are there any methods?
Hello Ayushi Gupta
Many anticancer regimens include compounds that bind to and/or modifies DNA. Fundamentally, drugs interact with DNA through two different ways, covalent and/or non-covalent modes. Covalent binders act as alkylating agents as they alkylate the nucleotides of DNA, while the non-covalent binders interact by three different ways: (i) intercalation, (ii) groove binding, and (iii) external binding (on the outside of the helix).
So, you could use various techniques to study the compound-DNA interactions such as electrophoresis, spectroscopy, mass spectrometry, viscosity measurements (viscometry), atomic force microscopy (AFM) and circular dichroism, and the effects of such interactions in the structure of DNA, providing some insights about the mechanism of drug action.
There are some references which I have attached that will help you to design your experiment without using cells.
https://pubmed.ncbi.nlm.nih.gov/17988854/
Article A paradoxical relationship between Resveratrol and copper (I...
https://www.scielo.br/j/bjps/a/mvZd6BVfqvdLf8sGNFGmKgL/?format=pdf&lang=en
Chapter Anticancer Drugs’ Deoxyribonucleic Acid (DNA) Interactions
Best.
We developed a number of active anticancer agents, and many of our advancements were based on chemical and physicochemical studies see the below publication.
Article Antitumor Sulfonylhydrazines: Design, Structure–Activity Rel...
Yes, there are methods available to test the anticancer properties of compounds without using cells or cell lines. One such method is using animal models, such as mice or rats. In these experiments, the compound of interest is administered to the animals, and then various parameters related to cancer growth, such as tumor size, metastasis, or overall survival, are measured.
Another method is utilizing tissue culture models, where tissue slices or explants from tumors or relevant organs are maintained ex vivo (outside of the body) and treated with the compound. This method allows for studying the effects of the compound on intact tissue structures without the need for isolated cells.
Additionally, computational modeling and simulation techniques can be employed to predict the potential anticancer properties of a compound based on its chemical structure and known interactions with biological targets.
These methods offer alternatives to traditional cell-based assays and can provide valuable insights into the anticancer potential of a given compound. However, it's important to note that each method has its own advantages and limitations, and the choice of method depends on factors such as the nature of the compound, research objectives, and available resources.
All the best
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