In the process of voltage clamp recording, cesium internal solution was commonly used in our laboratory. In general, we used high-chloride cesium internal solution to record mEPSC and mIPSC, but we used low-chloride cesium internal solution for PPR and evoked EPSC. Neither NMDA nor AMPA receptors are chloride channels, so why calculate the equilibrium potential of chloride?
Apostolos Mikroulis
If you are using high-Cl in the pipette, your IPSCs will have the same polarity as EPSCs. It's usually done to increase the amplitude of IPSCs when recording at physiological potentials (ca. -70 mV). If you want to distinguish EPSCs from IPSCs in that case you will have to either pharmacologically block one or the other, or rely on kinetics to classify them.
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