Hey Roberto,

I could not access the link you provided for the protocol. However, I coat my coverslips  with Poly-L-lysine (0.01% solution, Sigma) and let it dry under laminar flow. Once dried, I put mouse brain slice (sagittal or coronal, up to 400-micrometre thickness) on coated coverslips. It sticks very well and almost instantaneously to these coated coverslips.  I use them for electrophysiological recordings but you can give it a try. 

Hope it helps !

best luck,

Nand

Thanks for your reply, Nand. How much time do you leave the coverslips in poly-L-lysine before letting them dry? The main problem I find is my slices are horizontal and wrinkle easily when they are taken out of an aqueous solution, so it could be hard to stick them to dry coverslips without folding any part of the slice.

Best regards,

Roberto

Dear Roberto,

I apply just enough PLL (around 40 uL per 12 mm glass coverslip) to cover the maximum surface area and let it dry under laminar air flow to get thin PLL coat. I usually transfer brain slices using wide diameter plastic dropper and dab the excess solution with tissue paper to ensure unfolded slices. Once transferred into recording chamber it is superfused with aqueous buffer and usually doesn't come off coverslip that easily. Some colleagues also use a light weight metal wire mesh to keep the slice in place while recording. 

Best regards,

Nand

Dear Nand,

One of my colleagues showed me a small metal piece she uses and seems to work fine for my purpose, so I'll give it a try next time. Thanks for your kind replies.

Best regards,

Roberto