In an eye with Tyndall, does each dot correspond to an individual cell?

When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...

27 February 2021 6,326 2 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

How to interpret this FTIR spectrum of pyrolyzed hydrochar?

20 February 2021 2,840 3 View

Which statistical test is best to seperate out treatment effects meta-analysis?

I am doing a meta-analysis and am looking for some guidance on which statistical test will help me do the following. I want to understand the effect size a treatment has on a population. There...

16 February 2021 8,033 3 View

Sample preparation for ceramics i.e. iron silicate (fayalite), iron oxides, alumina, spinel mineral (hercynite)?

Recommendations in preparing ceramics for nanoindentation and in the process minimize introducing stresses from sample preparation i.e. polishing stage.

02 February 2021 6,211 3 View

How do I calculate a trend with 3 time points and 3 different groups with a small sample size in a longitudinal study?

Dear colleagues, I am working on an intervention study with 3 different groups of students. One group represents the intervention group and consists of a seminar with a practical phase and...

24 January 2021 8,051 4 View

Best method of removing au/pd coating from a mounted sample without removing the analysed material?

I have performed some SEM imaging on a conductive mounted sample which I also had to au/pd coat (10 seconds - so relatively thin coat). I would like to remove the coating and perform...

07 January 2021 2,490 4 View

PEI DNA in-vivo transfection concentration?

04 January 2021 6,908 7 View

Can XRD analyse a sample prepared for TEM?

I have samples that have been tested at high temperature (1160 and 1180 degrees) however, there are some phases formed that are not being picked up when using Raman. I am wondering if it would be...

03 January 2021 1,338 3 View

Stochastic optimization software?

Hello, I would like to know any experiences about stochastic optimization software or possibility to prepare it in Python for example. I am looking for an optimization process in Revenue...

26 November 2020 856 3 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Can I arbitrarily take a cycle number of 40 for the gene that was not expressed at all in qPCR results calculation?

I ran RT-qPCR for a control and a treated group and it appears that my gene of interest was not expressed at all in any of the subjects in the control group. So I was wondering if I could...

20 February 2021 6,243 10 View

Does anyone know if the Quantstudio 3 qPCR machine requires a passive reference when Using SYBR? I ran a qPCR and got the plot in the attached file.?

I know that my target is in my sample as I extracted it from cell culture myself. These plots show some amplification. The first one seems O.K for the first 36 cycles but then collapses at the...

14 February 2021 2,809 2 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View

Are there any exception like work hardening in metals elastic limit due to dynamic loading?

The samples made out of spring steel are taken from same lot. The specimens were shaped following heating to forging temperature - Forming- Hardening -Tempering methods. After storage of almost 6...

11 February 2021 952 6 View

Looking for help in writing my research proposal ?

Looking for help in writing my research proposal in the following areas : HRIS / HR / Change management / Remote work Please get in touch with me if that sounds like your expertise. Thank you very...

08 February 2021 5,434 4 View

What recipe of homemade lenti-virus concentrator do you use?

My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....

08 February 2021 5,965 1 View

Does anyone knows about a comercial software to record pressure and temperature during reaction in an Autoclave?

Dear all: Currently I am working with polymerization reaction in a reactor. For this, I need to control temperature, pressure, agitation speed etc. For calculation of rate of polymerization, I...

07 February 2021 355 2 View

Having trouble with qPCR amplification plots. My curve's never seem to reach a plateau. Any ideas?

The parameters of the curve all seem acceptable, typically reactions results are above 0.9852, -3.1 to -3.6 slope, and 90-110% reaction efficiency. The results seem to be what I would expect from...

04 February 2021 1,138 3 View