How can I fill hourly temperature gaps?

I have hourly temperature data from 2 Automatic Weather Stations located less than 500 m distance. I would like to fill the gaps in both series using the data of the other AWS. Which methodology...

03 May 2020 3,758 7 View

As a social scientist anticipating new religious research on COVID-19, what should quantitative & qualitative sociologists of religion be asking?

COVID-19 is already accentuating interesting religious phenomena. Churches closing, beliefs regarding healing, trust in science are the kinds of issues we might ask regarding the relationship...

11 April 2020 6,231 56 View

What are the best tools to create Feedback Forms related to Excel sheets?

I want to gather feedback from the implementation of processes in order to optimize them and I was wondering what tools other than 'google forms' could allow me to implement them in a formal and...

23 December 2019 8,304 3 View

Is it possible to buy LEDs with their Center Wavelength emission (or ideally with the full emission irradiance spectra)?

05 April 2016 4,177 6 View

I need a reliable UV-Vis radiation device to measure accurate reaction kinetics of as photochemical reaction?

11 March 2016 5,568 2 View

Is there anyone who has experience with the program simulation engines LOTUS ENGINE?

03 January 2015 4,216 2 View

How do the surface of the areas defined by the parallels and meridians change with latitude?

What is the mathematical function that determines the area defined between two parallels and two meridians depending on latitude?

05 June 2014 2,850 4 View

How does the solar radiation change with latitude?

What is the mathematical function that determines the solar radiation received by the area defined by two parallels and two meridians depending on latitude? How can the tilt of the axis of...

05 June 2014 1,814 8 View

Does anti-matter exist in our universe? Is positron a reality?

If positron is a reality, then, if we bombard the positron with electrons, is a black hole formed or into which form of energy is it transformed?

13 April 2014 7,869 9 View

I was wondering if it is possible to perform a calculation in Gaussian 09 using MMFF94 forcefield?

In the manual only Amber, Dreiding, and UFF Molecular Mechanics Methods are described. Any ideas if this is possible?

15 January 2014 7,915 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How can we limit artifacts caused by rotation in fiber photometry?

We are trying to run fiber photometry experiments for long time scales, but we get these sinusoidal artifacts that come up. We think they may be caused by twisting of the fiber optic subject...

25 February 2021 568 1 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View