How can I fill hourly temperature gaps?

I have hourly temperature data from 2 Automatic Weather Stations located less than 500 m distance. I would like to fill the gaps in both series using the data of the other AWS. Which methodology...

03 May 2020 3,758 7 View

As a social scientist anticipating new religious research on COVID-19, what should quantitative & qualitative sociologists of religion be asking?

COVID-19 is already accentuating interesting religious phenomena. Churches closing, beliefs regarding healing, trust in science are the kinds of issues we might ask regarding the relationship...

11 April 2020 6,231 56 View

What are the best tools to create Feedback Forms related to Excel sheets?

I want to gather feedback from the implementation of processes in order to optimize them and I was wondering what tools other than 'google forms' could allow me to implement them in a formal and...

23 December 2019 8,304 3 View

Is it possible to buy LEDs with their Center Wavelength emission (or ideally with the full emission irradiance spectra)?

05 April 2016 4,177 6 View

I need a reliable UV-Vis radiation device to measure accurate reaction kinetics of as photochemical reaction?

11 March 2016 5,568 2 View

Is there anyone who has experience with the program simulation engines LOTUS ENGINE?

03 January 2015 4,216 2 View

How do the surface of the areas defined by the parallels and meridians change with latitude?

What is the mathematical function that determines the area defined between two parallels and two meridians depending on latitude?

05 June 2014 2,850 4 View

How does the solar radiation change with latitude?

What is the mathematical function that determines the solar radiation received by the area defined by two parallels and two meridians depending on latitude? How can the tilt of the axis of...

05 June 2014 1,814 8 View

Does anti-matter exist in our universe? Is positron a reality?

If positron is a reality, then, if we bombard the positron with electrons, is a black hole formed or into which form of energy is it transformed?

13 April 2014 7,869 9 View

I was wondering if it is possible to perform a calculation in Gaussian 09 using MMFF94 forcefield?

In the manual only Amber, Dreiding, and UFF Molecular Mechanics Methods are described. Any ideas if this is possible?

15 January 2014 7,915 2 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

How can we limit artifacts caused by rotation in fiber photometry?

We are trying to run fiber photometry experiments for long time scales, but we get these sinusoidal artifacts that come up. We think they may be caused by twisting of the fiber optic subject...

25 February 2021 568 1 View

How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

How could the concentration from RNA extraction is really low?

Hello everyone, currently, I'm trying to do RNA extraction from seeds of Arabidopsis. I'm using the protocol below: RNA isolation from siliques, dry seeds, and other tissues of... I use about...

17 February 2021 7,583 4 View

Hi, can anybody help me with the suggestions how can I amplify a stress induced gene of 1107 bp?

I am trying to amplify the TGA1 (Transcription factor 1 ) of Arabidopsis ,which is 1107 bp. I have treated the plants with Salycylic solution over night and extracted the RNA and make cDNA. I...

17 February 2021 7,802 2 View

How do I check the quality of my viral RNA prior to metagenomic sequencing?

I am doing metagenomic sequencing without enrichment for an RNA virus. After my RNA is extracted from the blood I am doing library prep with the NEBNext Ultra directional RNA library prep kit for...

16 February 2021 5,101 2 View

How to remove the species data from rda data frame in R (only remain the sites/samples point)?

Dear everyone, When I using the rda function in r, I met this question. As you can see in the figure, there are a lot of deep blue points which refers to the species. But I would like remain...

15 February 2021 8,982 2 View

RNA concentration: Nanodrop vs Agilent 2100 Bioanalyzer?

Hi guys. I make RNA by in vitro transcription and do cell transfection afterwards. I analyze RNA using Nanodrop and Bioanalyzer. But I found the concentration of RNA measured by these two machines...

05 February 2021 292 3 View

Apart from MOFA what other software is available for analysis of a multi-omic study?

Study bringing together genomics, proteomics etc together

02 February 2021 2,856 2 View