Strong and extreme winds in the Arctic?

Hello everybody! Looking for a good source of information on strong and extreme winds in the Arctic (monthly values, annual extremes etc.). The form of maps (with isolines) will be most...

25 January 2021 8,984 4 View

No title

Dear Colleagues, I need your help. In book (can be found in Internet, free) Todhunter, I., & Pearson, K. (1893). A History of the Theory of Elasticity and of the Strength of Materials (Vol....

26 December 2020 433 1 View

Combine several datasets?

Dear colleagues, I would appreciate your advice on the following issue. Let's say, first I had investigated expression of Prot1 and Prot2 in tumor tissue, it's association with relevant clinical...

31 October 2020 4,875 2 View

Does the quantum vacuum have analogies in mathematics?

Take, for example, such a concept as a minimum flow, that is, a gradient vector field, the level surfaces of which are the minimum surfaces. Then the globally minimal flow, evolving to an...

13 September 2020 8,593 13 View

Can AI be emotionally intelligent (whatever AI, emotional, or intelligent may mean to you)?

Can AI be emotionally intelligent (whatever AI, emotional, or intelligent may mean to you)? Research addresses machine recognition and modeling of human emotional expression, including the...

11 September 2020 5,158 5 View

Where was Fischerella sp. PCC 9339/Fischerella musciola (UTEX1829) isolated?

Dear colleagues, I am currently comparing some genomic features in different representatives of Fischerella genus isolated from different eco-types. Unfortunately, neither PCC nor UTEX collection...

11 July 2020 4,003 12 View

Will DNA degradate if left over weekend during tissue lysis in extraction methods ?

Hi, Question occured while discussing with colleges if it's safe to leave tissue lysis over weekend, because there are no problems to left overnight which can even be written in some extraction...

09 July 2020 4,084 4 View

Are these claims about compactification true?

1. Do I understand correctly that closed curves on a 3-dimensional torus are represented as closed ribbons lying freely (with the possibility of inverting) on a classical torus? 2. Do I understand...

20 May 2020 799 3 View

Possible optimization algorithms for fitting a nonlinear model with no analytic solution (contains integrals that can only be solved numerically)?

I would be grateful for suggestions to solve the following problem. The task is to fit a mechanistically-motivated nonlinear mathematical model (4-6 parameters, depending on version of assumptions...

14 April 2020 2,117 12 View

How can I design a PBG substrate microstrip antenna in Matlab?

I'm using antennaDesign tool box of Matlab to design a insetfed Microstrip antenna. Now I want to compare the results with the same antenna, but with a PBG substrate. How can I do this?

10 April 2020 7,257 4 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View