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Questions related from Igor V Gorbenko
Dear colleagues! We are trying to isolate mitochondria from conifer plants. The regular Percoll step gradient isn't working due to the percularities of the material, so we want to try mixed...
23 June 2023 1,941 0 View
We isolated mitochondria from freshwater amphipods and found that being disperged throughout the media in a tube - mitochondria soon starts to form flakes and aggregates. We thimk this is also...
17 June 2023 2,741 0 View
Dear colleagues! We are currently conducting protocol adaptation for isolation of mitochondria from freshwater amphipods. The isolation medium was from the protocol of mitochondria isolation from...
16 June 2023 5,211 0 View
Greetings! I have an issue that drives me crazy this evening... I have a list of gene vectors, downregulated in different transgenic plants and I want to make a Venn diagram to visualize it and...
11 June 2023 2,079 4 View
Dear colleagues! I've tried to estimate the histone variants enrichment of several arabidopsis genomic sites but every time my enrichment result looks like the added screenshot. What am I doing...
31 March 2023 5,687 1 View
We try to use RepeatMasker for de novo annotation of Arabidopsis transposable elements but it seemes that DFAM database don't have plant-specific TE hmm profiles... and we don't have the access...
07 February 2023 4,470 0 View
Good day! I have a list (~10000) of unique DNA sequences about 10-20 bp. I want to find out if they could evolve from one or several sequences, or emerged independently. Some of the sequences...
02 February 2023 5,296 1 View
Good day! I'm using the BioRad PROTEAN II XI chamber and gradiend polyakrylamide gel - 3.3%-20%. I used to run the gel overnight at 105-110 V, but this time got the "Error no load" after ~6 hours...
24 November 2022 8,586 4 View
Dear colleagues! We plan to isolate mitochondria from freshwater amphipods, but didn't find any methods in literature - the closest found was the method of isolation from whiteleg shrimp...
02 November 2022 6,705 4 View
What package fo you use to visualize for example UTRs, exons, introns and read coverage of a single gene? Or a plasmid map in linear form?
12 October 2022 7,258 5 View
Good day! I need to elute proteins from polyacrylamide gel slices after BN-PAGE electrophoresis. The porblem is that the gel slices are relatively big - all the lane fragment upper from 1000 kDa...
27 December 2021 7,535 4 View
Good day! I've found many different western blot protocols and it is mostly recommended to prepare primary antibody hybridization solution with blocking buffer (5% skim milk in TBST) and to...
27 October 2021 9,790 3 View
Good day! I have some predicted sequences that was not previously annotated in plant genome. Than It was found that it's possible to BLAST these sequences using RNAseq data found in ncbi SRA...
12 November 2019 6,103 3 View
Good day! I have a data frame of LogFC of differential expression data of several genes in trangenic plants with overexpression/knockout of one particular gene (and overexpression is different),...
30 April 2019 3,684 4 View
