Recently the isolation of exosomes from serum using a simplified protocol based on acetone precipitation was reported (Gallart-Palau Scientific Reports 5, Article number: 14664, 2015). i would like to know if exosomes isolated following that procedure are functional when incubating them with cells, so they can trigger any observable effect in transcription or translation of proteins
Dear Asier,
The indicated paper was published in September 30, 2015. We should wait some time to see research evolved using the exosomes isolated by this novel method.
Rafik
Thank you very much for your replies. I believe it is really difficult to preserve integrity of exosomes in such a high concentration of acetone. Maybe most of surface proteins are not affected but I highly doubt that cargo remains completely unaffected even if extracellular vesicles look intact by cryo-electron microscopy
I never tried to precipitate EVs from serum or cell culture media by PROSPR method. I do believe that proteins (surface and cargo) will get denature. Acetone can easily penetrate the EV membrane. On the other side, this method will be good for EVs number and structural analysis. In future, I am planning to test this method in my cell culture system. I will keep you updated..........thanks!!
Anyone has any updates on this exosome isolation technique? Thank you
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