Asier Galan

3 Questions 4 Answers 0 Followers

Questions related from Asier Galan

What is the best procedure to enrich phosphoproteins/phosphopeptides from serum/plasma?

I plan to use filters to get the fraction below 25kDa from serum proteins, then perform digestion and enrichment using TiO2 magnetic beads ( avoiding albumin depletion that can result in low...

07 May 2016 3,187 3 View

Are the exosomes isolated from serum by PROSPR precipitation functional?

Recently the isolation of exosomes from serum using a simplified protocol based on acetone precipitation was reported (Gallart-Palau Scientific Reports 5, Article number: 14664, 2015). i would...

30 November 2015 9,936 4 View

What is the best protocol for isolating Mitochondria-Associated Membrane (MAM)

Many cellular processes require the proper cooperation between mitochondria and the endoplasmic reticulum (ER). Several recent works show that their functional interactions rely on dynamic...

02 July 2014 8,415 4 View