Asier Galan

5 Questions 6 Answers 0 Followers

Questions related from Asier Galan

Which antibody is more effective to bind/capture exosomes when immobilized on magnetic bead support?

I am coating epoxy magnetic beads (Dynabeads TM M-270 Epoxy) with anti-CD63 antibody to bind/capture and purify exosomes from urine and serum. We are having some mixture of particle sizes, so we...

10 November 2017 6,528 0 View

Is the composition of ESI emitter significant for phosphoprotein/phosphopeptide analysis by LC-MS/MS?

I am going to use LC-MS/MS for the characterization of protein phosphorylation and I wonder if the tip of the ESI emitter can bind phosphopepides if it is metallic instead of made of silica

21 September 2016 6,930 3 View

What is the best procedure to enrich phosphoproteins/phosphopeptides from serum/plasma?

I plan to use filters to get the fraction below 25kDa from serum proteins, then perform digestion and enrichment using TiO2 magnetic beads ( avoiding albumin depletion that can result in low...

07 May 2016 3,218 3 View

Are the exosomes isolated from serum by PROSPR precipitation functional?

Recently the isolation of exosomes from serum using a simplified protocol based on acetone precipitation was reported (Gallart-Palau Scientific Reports 5, Article number: 14664, 2015). i would...

30 November 2015 9,962 4 View

What is the best protocol for isolating Mitochondria-Associated Membrane (MAM)

Many cellular processes require the proper cooperation between mitochondria and the endoplasmic reticulum (ER). Several recent works show that their functional interactions rely on dynamic...

02 July 2014 8,443 4 View