How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View

Is it possible to obtain a negative cronbach alpha?

Good evening everyone. If a questionnaire has 4 factors measuring different constructs as for example CAMI questionnaire measuring attitudes toward the mentally ill that has four factors:...

18 February 2021 3,223 3 View

Where can I find a reliable and updated methodology related with the design of spiral heat exchangers?

I need to design an spiral heat exchanger, buy I don't know what reference or bibliography (preferably a book) should I consult in order to get an updated and reliable methodology related with...

14 February 2021 7,910 3 View

Can a mRNA be degraded but still observe a protein over-expression?

Hello there, I'm currently trying to over-express a gene by transfecting the cells with a pcDNA6 plasmid containing my target gene. When I measure the mRNA for my gene of interest, the levels are...

03 February 2021 4,112 4 View

How can I do the bang's blinding index and james blinding index?

Hi! I would like to know with which statistical software the bang blinding index and the James blinding index and how to do it. Thanks

09 December 2020 1,363 2 View

What is the main difference of diluting HCL in methanol as opposed to water?

I am hoping to determine the mass per unit area (as per DIN 50938) of a black oxide coating. I use water to achieve the required 1mol/l hydrochloric acidic solution, but a fellow lab had used...

06 December 2020 4,806 1 View

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Greetings to all. I have some questions about setting up DFT calculations in Gaussian software (I am a beginner in these matters), and trying to calculate the energy of the first excited triplet...

06 December 2020 3,504 4 View

What is exact impact of pH on preparation method of nanoparticles? why some nanoparticles are synthesized in ACIDIC pH while others in alkaline pH?

I am trying to prepare Cerium oxide nanoparticles. literature suggested the acidic pH of the reaction. what is the reason? what is the mechanism if so

28 November 2020 8,825 2 View

Estimate / Predict remaining useful life of a bearing without data of previous failure?

Hello, I am trying to write a program in Matlab to estimate the remaining useful life of a bearing without data of a previous failure. Do you think this is possible? I was thinking to use the...

27 November 2020 9,863 3 View

Why is cronbach's alpha sometimes calculated for non-unidimensional questionaries?

I've recently come across with several published articles where questionaries composed of more than one section (where every section is meant to gather data for separate variables/dimensions of...

11 November 2020 1,805 7 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

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25 February 2021 5,713 1 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

Fictitious Boundary Meshing Method (FBM) in Commercial FEA software?

Is it possible to implement FBM in a commercial FEA software package, such as FEMLAB, COMSOL, or open source software like Moose?

22 February 2021 2,968 1 View

Are there packages (R or Python) for simple models (bucket?) to assess soil water content?

Hi everyone. I am researching droguht effects on soil water drought. I am looking for already implemented functions in R or Python to estimate soil water content from precipitation,...

21 February 2021 2,084 1 View

How reliable are degrees of freedom for lme mixed models in nmle package?

I am running mixed models analyses because I have participants nested in groups. Across the different analyses, I have very different degrees of freedom in the lme output with nlme package (and...

09 February 2021 6,767 3 View

Which software is the best to model a non-linear concrete (ABAQUS, ANSYS,ADINA, ATENA, etc.)?

What is the best finite element modeling software packages for modeling non-linear and fracture analysis of CONCRETE? I've had some with ABAQUS standart/explicit, but I would like to learn if...

27 January 2021 330 6 View

How plot results of membership function in R?

I am using the FRBS package, I developed the model without learning data and I use the plotMF function of this package to see the membership function of every input and output variables. I got the...

22 January 2021 2,668 3 View