URGENT: How to eliminate mold contamination from brain tissue slices?

03 November 2020 8,357 2 View

Non extruded nanoparticles loaded with monoclonal antibody. How to get the highest encapsulation efficiency and small size?

Hello I am new in the nanoparticles research field. My master project includes the development of a nonextruded LysoPS:DMPC nanoparticle loaded with an antibody. What will you recommend to get...

31 August 2019 8,542 4 View

Combining Immunohistochemistry and Immunofluorescence in the same sample slide?

Has anyone tried combining Immunohistochemistry using alkaline phosphatase technique and Immunofluorescence in the same sample slide?

31 January 2019 2,008 3 View

Do you know any methods to obtain microbial inoculations of native forest soil? Could you please send me a bibliography about it? Thank you

Biologists, agronomists, soil researchers

15 May 2017 9,059 2 View

Normal epithelial ovarian cell line?

We are looking for a non-transformed immortalised ovarian cell line to use as a control in a dose-response experiment. We have found this line T1074 in abmgood. Do someone have this cell...

07 May 2017 3,065 2 View

Myeloid derived macrophage markers (from murine bone marrow)?

18 August 2016 4,256 4 View

Specific for/rev mouse II26 primer sequences?

Looking for qRT-PCR (Sybr method) Il26 primer sequences (for and rev) for mouse samples. Does anyone have a good suggestion for good and specific primer sequences?Thank you!

26 April 2016 6,344 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Found that there was a multiple band when amplifiying targeted gene ?

i few days ago amplified specific gene using PCR with specific primers, but there was a multiple band in electrophoregram i found. out of 10 sample amplified, there was multiple band and there was...

18 February 2021 5,131 5 View