Are CSCs expressed in all cancer patients? Are there any diagnosis?
Dear colleagues, I would like send DNA samples for sequencing to company outside my stay country for gene sequencing service. The company that will do the sequence service requested me to send...
04 May 2015 3,091 14 View
Hello everyone, I isolated 23 bacterial isolates from UTI samples. I determined the species and subspecies name by technique available in my Lab, but I unable to assign strain name. I do not...
02 March 2015 5,107 36 View
Hello everyone, I would like to study the protein profile of TB, so I have to transfect DNA of M. TUBERCULOSIS with a type of cell line. Kindly your Opinions and recommendations. Thank you
31 December 2014 7,713 4 View
Hello everyone,I would like you state an advise for me. I have programming and scripting skills, so I can develop Bioinformatics tools based on available modules and improve new codes, but while...
11 December 2014 668 6 View
I got 97% matching in alignment for two genes " SUl-1 & blaCTX-M-15" isolated from Klebsiella pneumoniae ssp pneumoniae, so is it possible to get publish in gene bank database? Then I submitted 2...
04 May 2014 5,942 5 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View