How many reads are "sufficient" to characterize free living nitrogen fixation microbial communities in soil?

I plan to do nifH targeted NGS (most likely paired end reads with MiSeq) to study free living nitrogen microbial communities in different tillage management system soil samples. I see as much as...

09 October 2017 1,715 1 View

15N2 method for Nitrogen fixation and would like to share your experience?

Hi, We are working on 15N2 method to determine Nitrogen fixation by free living in Soil. However, the difference in between samples of natural abundance (NA) and Enriched (EN) by 15N2 is very very...

09 October 2017 4,744 3 View

Does incubation period influence 15N2 isotope results

Hi, I tried 15N2 method to determine free living bacterial activity in soil samples at 2d, 4d and 8d incubation period. However, i have did not find considerable activity. Does incubation period...

06 July 2017 3,126 4 View

How to Convert ul/l to umol/g?

Please let me know how to convert ul/l to umol/gram gas samples for 1000mL jar volume. Thanks..

31 December 2016 1,581 0 View

Determine nitrogen fixation by free living in soil?

Hi, I spent lots of time to figure out ARA method to determine nitrogen fixation by free living in soil. However, I am not getting significance difference between ethylene peaks of control (no...

10 November 2016 6,774 3 View

How to calculate ul/l C2H4 in per gram Soil?

Hello, I need to calculate ul/l C2H4 in per gram soil. For instance, I got 7.098ul/l C2H4 in 1040mL jar at 250g of Soil. How to calculate this C2H4 in per gram soil.

10 November 2016 8,238 0 View

Does soil became Anaerobic in 15 days long incubation?

09 October 2016 7,974 2 View

Mothur data error?

test.trim.fasta does not showing any data content while using Mothur. Please advice..

09 October 2016 7,683 2 View

How to prepare soil samples for DNA Isolation?

09 October 2016 4,154 1 View

Rhizobia to inoculate in to the soil?

08 September 2016 4,185 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View