14 Questions 12 Answers 0 Followers
Questions related from Manoj Kumar
Hi, We are working on 15N2 method to determine Nitrogen fixation by free living in Soil. However, the difference in between samples of natural abundance (NA) and Enriched (EN) by 15N2 is very very...
10 October 2017 4,794 3 View
I plan to do nifH targeted NGS (most likely paired end reads with MiSeq) to study free living nitrogen microbial communities in different tillage management system soil samples. I see as much as...
10 October 2017 1,753 1 View
Hi, I tried 15N2 method to determine free living bacterial activity in soil samples at 2d, 4d and 8d incubation period. However, i have did not find considerable activity. Does incubation period...
07 July 2017 3,170 4 View
Hi, Could you suggest me the primers set for nifH that can cover the good sequence variability of the nifH gene and capture the full diversity. Thanks
05 May 2017 6,404 2 View
Please let me know how to convert ul/l to umol/gram gas samples for 1000mL jar volume. Thanks..
01 January 2017 1,620 0 View
Hello, I need to calculate ul/l C2H4 in per gram soil. For instance, I got 7.098ul/l C2H4 in 1040mL jar at 250g of Soil. How to calculate this C2H4 in per gram soil.
11 November 2016 8,283 0 View
Hi, I spent lots of time to figure out ARA method to determine nitrogen fixation by free living in soil. However, I am not getting significance difference between ethylene peaks of control (no...
11 November 2016 6,814 3 View
test.trim.fasta does not showing any data content while using Mothur. Please advice..
10 October 2016 7,722 2 View
Hi, I will use PowerSoil DNA Isolation Kit for bacterial study. I wanted to know before use kit how to prepare soil samples. Currently, these are frozen in -20C. how long soil should keep on room...
10 October 2016 4,192 1 View
Please advise, I need to keep my soil sample for long incubation (15 days) to determine nitrogen fixing using acetylene reductase method. In between does any chance to became soil Anaerobic.
10 October 2016 8,015 2 View
Dear Researcher, please let me know can bulk density of soil determine in the lab with air dry soil packed in to ring? Thanks
09 September 2016 7,536 11 View
Could let know the quantity of rhizobias (are in liquid) to inoculate in to the 100g soil for determining nitrogen fixation?
09 September 2016 4,228 2 View
Hello, I spent lots of time to figure out ARA method to determine nitrogen fixation by free living in soil. However, I am not getting significance difference between ethylene peaks of control (no...
09 September 2016 4,363 6 View
Can any one suggest accurate protocol for ARA method for free living in soil.
08 August 2016 3,058 2 View
