Mothur data error?

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How many reads are "sufficient" to characterize free living nitrogen fixation microbial communities in soil?

I plan to do nifH targeted NGS (most likely paired end reads with MiSeq) to study free living nitrogen microbial communities in different tillage management system soil samples. I see as much as...

09 October 2017 1,773 1 View

15N2 method for Nitrogen fixation and would like to share your experience?

Hi, We are working on 15N2 method to determine Nitrogen fixation by free living in Soil. However, the difference in between samples of natural abundance (NA) and Enriched (EN) by 15N2 is very very...

09 October 2017 4,814 3 View

Does incubation period influence 15N2 isotope results

Hi, I tried 15N2 method to determine free living bacterial activity in soil samples at 2d, 4d and 8d incubation period. However, i have did not find considerable activity. Does incubation period...

06 July 2017 3,186 4 View

Appropriate primers set for nifH?

Hi, Could you suggest me the primers set for nifH that can cover the good sequence variability of the nifH gene and capture the full diversity. Thanks

04 May 2017 6,419 2 View

How to Convert ul/l to umol/g?

Please let me know how to convert ul/l to umol/gram gas samples for 1000mL jar volume. Thanks..

31 December 2016 1,640 0 View

Determine nitrogen fixation by free living in soil?

Hi, I spent lots of time to figure out ARA method to determine nitrogen fixation by free living in soil. However, I am not getting significance difference between ethylene peaks of control (no...

10 November 2016 6,832 3 View

How to calculate ul/l C2H4 in per gram Soil?

Hello, I need to calculate ul/l C2H4 in per gram soil. For instance, I got 7.098ul/l C2H4 in 1040mL jar at 250g of Soil. How to calculate this C2H4 in per gram soil.

10 November 2016 8,299 0 View

Does soil became Anaerobic in 15 days long incubation?

Please advise, I need to keep my soil sample for long incubation (15 days) to determine nitrogen fixing using acetylene reductase method. In between does any chance to became soil Anaerobic.

09 October 2016 8,033 2 View

How to prepare soil samples for DNA Isolation?

Hi, I will use PowerSoil DNA Isolation Kit for bacterial study. I wanted to know before use kit how to prepare soil samples. Currently, these are frozen in -20C. how long soil should keep on room...

09 October 2016 4,206 1 View

Determine bulk density in the lab?

Dear Researcher, please let me know can bulk density of soil determine in the lab with air dry soil packed in to ring? Thanks

08 September 2016 7,553 11 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...

10 August 2024 4,005 2 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Is Galaxy.org good to use for research for analyzing data and for publication?

Hello all, I wanted to know, can I use galaxy (USA, Europe or Australia) platform for analyzing the shotgun data, and can it be used for publication purpose as well? Thanks :)

06 August 2024 6,610 4 View

Do experts have journals in the field of artificial intelligence and big data that are not indexed by SCI or EI?

05 August 2024 8,836 2 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

How can I interpret the data without the need of solving it manually?

How can I interpret the data gathered without solving?

03 August 2024 9,054 3 View

Why can't academics earn the money they deserve?

Only Journals make money from the articles we have worked on for years. Academics do not earn money from their refereeing. Then shouldn't the solution be a system in which academics can earn...

01 August 2024 6,469 6 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

How Do Project Data Analytics and AI Advance Quality 4.0 in Construction Project Management?

As the construction industry advances, the integration of Project Data Analytics and Artificial Intelligence (AI) is becoming increasingly crucial in project management. These technologies are...

31 July 2024 6,484 1 View

Shafagat Mahmudova

Dear Manoj Kumar,

Look the link. May be useful.

http://www.hmpdacc.org/doc/16SrRNA_GeneCuration_SOP.pdf

http://www.opiniomics.org/a-mothur-tutorial-what-can-we-find-out-about-the-horse-gut-metagenome/

Regards, Shafagat

Manoj Kumar

Hi Shafagat, Thanks for information. While following Mothur MiSeq SOP i did not get, split and again making groups. I have a file of FASTQ. I tried process by the following link..https://www.jove.com/video/51709/next-generation-sequencing-of-16s-ribosomal-rna-gene-amplicons.

I am trying SRA experiment file (ERX1425102) with Mothur.