How many reads are "sufficient" to characterize free living nitrogen fixation microbial communities in soil?

I plan to do nifH targeted NGS (most likely paired end reads with MiSeq) to study free living nitrogen microbial communities in different tillage management system soil samples. I see as much as...

09 October 2017 1,715 1 View

15N2 method for Nitrogen fixation and would like to share your experience?

Hi, We are working on 15N2 method to determine Nitrogen fixation by free living in Soil. However, the difference in between samples of natural abundance (NA) and Enriched (EN) by 15N2 is very very...

09 October 2017 4,744 3 View

Does incubation period influence 15N2 isotope results

Hi, I tried 15N2 method to determine free living bacterial activity in soil samples at 2d, 4d and 8d incubation period. However, i have did not find considerable activity. Does incubation period...

06 July 2017 3,126 4 View

Appropriate primers set for nifH?

04 May 2017 6,353 2 View

How to Convert ul/l to umol/g?

Please let me know how to convert ul/l to umol/gram gas samples for 1000mL jar volume. Thanks..

31 December 2016 1,581 0 View

Determine nitrogen fixation by free living in soil?

Hi, I spent lots of time to figure out ARA method to determine nitrogen fixation by free living in soil. However, I am not getting significance difference between ethylene peaks of control (no...

10 November 2016 6,774 3 View

How to calculate ul/l C2H4 in per gram Soil?

Hello, I need to calculate ul/l C2H4 in per gram soil. For instance, I got 7.098ul/l C2H4 in 1040mL jar at 250g of Soil. How to calculate this C2H4 in per gram soil.

10 November 2016 8,238 0 View

Does soil became Anaerobic in 15 days long incubation?

09 October 2016 7,974 2 View

How to prepare soil samples for DNA Isolation?

09 October 2016 4,154 1 View

Rhizobia to inoculate in to the soil?

08 September 2016 4,185 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View

Is there any Machine Learning/AI algorithm that lets you predict with incomplete features without missing value imputation process?

For instance, the model is trained with A,B,C,D,E features to predict F target. Then, I want to make prediction on a new data, but with only A,C,D,E is known without doing missing value imputation...

28 February 2021 1,556 6 View