Create a Bland-Altman plot for method comparison when there is more than one measurement per subject with each laboratory method.
The Bland-Altman plot or difference plot, is a graphical method to compare two measurements techniques. In this graphical method the differences (or alternatively the ratios) between the two techniques are plotted against the averages of the two techniques. Alternatively (Krouwer, 2008) the differences can be plotted against one of the two methods, if this method is a reference or "gold standard" method.
Horizontal lines are drawn at the mean difference, and at the limits of agreement, which are defined as the mean difference plus and minus 1.96 times the standard deviation of the differences. If the differences within mean ± 1.96 SD are not clinically important, the two methods may be used interchangeably.
The plot is useful to reveal a relationship between the differences and the averages, to look for any systematic biases and to identify possible outliers.