So I have been using Fugene HD for my transfections with AGS and haven't had any major issues except that I can't perform large cell volume transfections due to cost and availability of Promega's Fugene HD. I was wondering if there were others here who've had success transfecting AGS cells in either suspension or with electroporation as either will allow me to transfect a decent amount of AGS cells that can be directly grown in larger culture plates and not use a huge amount of transfection reagents in the process. Really want to transfect around 100mm culture dish worth of AGS but really don't wanna think about the cost and amount of Fugene HD that this will use (will handle at least 6 samples).
Any help will be greatly appreciated.
AGS isn't a suspension cell line, so using lipofection is generally the route to go. Several reagents are out on the market, this one (https://altogen.com/product/ags-transfection-reagent-gastric-adenocarcinoma/) is specific to AGS cells and should give you high efficiency. If you're needing higher volumes of transfected cells, just culture them afterwards, and if you had high efficiency then you'll have a stable population of cultured cells.
Hi Kwangsun,
Carcinoma cell lines, such as HeLa and B16F10, can be efficiently transfected with the cationic lipid-based transfection reagent SAINT-MIX. Transfection with SAINT-MIX can be performed in the presence of serum and is non-toxic.
http://www.synvolux.nl/
The price for 1 ml SAINT-MIX is 99 euro and discounts for large quantities are possible. If you want more information, just send an email to [email protected].
Best regards
Wanda
Dear Kwabgsun,
I can recommend DreamFect Gold for transfecting your gastric adenocarcinoma
You can find results and publications here
http://www.ozbiosciences.com/transfection-dna/19-dreamfect-gold-transfection-reagent.html
If you need a free sample let us know at [email protected]
Good Luck
Olivier
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