Which ATP optical sensors for presynaptic terminal would be good?

08 June 2022 0 9K Report

I am planning to measure mitochondrial ATP synthesis in presynaptic terminals of cultured neurons. I searched for some indicators like FRET-based ATeam, ATP/ADP ratiometric sensor, luciferase-based luminescent sensor, and ATPSnFR. Among these, only the luminescent sensor (Rangaraju, 2014) was verified for usage in a single presynaptic terminal scale while others were used in a whole-cell scale. I would like to hear any experience from you to decide on a sensor in neurons. Any other sensor except I mentioned is welcome.

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