this cell is iPSC derived mesoderm.
I dont know what makes my cells diying...
please tell me What kind of phenomenon does it look like during the process of dying? I'd like to get a clue as to why.
looks like bacterial contamination
I'm doing a Western blot and haven't seen any protein for two months. After transferring, the marker is visible, but when I detect the protein once, wash it, and try to detect another protein the...
10 June 2024 6,716 7 View
Hello. I am trying to do contact-separation mode TENG. I have been following , and I am suffering to calculate the Open circuit voltage (Voc). I made geometry and set the materials setting all...
26 May 2024 2,645 0 View
I want to calculate e- number using K-L plot. I usually use current density when potential is 0.5V, 0.4V, 0.3V. But in the LSV data, if I use current density of same potential range, the e-...
29 April 2024 6,167 0 View
I am studying about triboelectricity, and I have a question why some open circuit voltages some out as single-peak and some output voltages comes out as double-peak. 1. The first one has only one...
03 September 2023 5,044 1 View
Hello, i'm struggling to improve EQE of QDLED. now, i'm using commercial CdSe QD. HIL, HTL, and ETL is PEDOT:PSS, PVK, and Al doped ZnO. respectively. to make balance of charge, i designed thin...
30 July 2023 6,810 1 View
Hi, I have some question about adapting hot carrier injection model in NOR flash unit cell. My NOR Flash unit cell is made of Floating gate Storage Node. I want to program the cell with HCI(Hot...
28 December 2022 5,227 1 View
Before passaging, and freezing, this ipsc passage's sum was 63 after culturing, I passaged and froze. First, I found that the transferred cell was not attached on matrigel plates (0.5mg/plate) I...
05 December 2022 8,015 2 View
I am planning to measure mitochondrial ATP synthesis in presynaptic terminals of cultured neurons. I searched for some indicators like FRET-based ATeam, ATP/ADP ratiometric sensor,...
07 June 2022 8,874 0 View
Many papers suggest just dissolving 0.1% of SDS in water. I wonder if it is not necessary to use a buffer or if the pH of the solution should be adjusted according to the protein pH of the...
12 July 2021 4,427 1 View
Which cell line offers the highest transfection efficiency? in Multiple myeloma or Neuroblastoma. I heard in multiple myeloma RPMI-8226 and U266 is hard to transfect. and Neuroblastoma IMR-32 is...
23 June 2021 7,356 2 View
I'm using Dorsomorphin (C24H25N5O) as one component in dual SMAD inhibition of pluripotent stem cells to differentiate into neurons alongwith SB431542. Now as to solubilizing 10mg SB in DMSO for...
11 July 2024 4,832 0 View
I’m currently reprogramming my fibroblast cells using Simplicon RNA OKSG-cMyc TagRFP Kit and after 13 days, these colony/aggregates started to show up in all of my wells. I have 6 wells, 2 wells...
07 July 2024 9,034 2 View
I saw the iPSC about a week after holding the stock (12 well and 3 stocks) and there were very few cells attached, and some cells were differentiated. I wonder what the problem is. The plate is...
12 June 2024 6,087 0 View
This shape gradually spreads. Even if you install new cells again, it will change like this at some point. Has anyone experienced the same thing as me? I tried using Matrigel again by aliquot it....
12 June 2024 7,269 5 View
Hello everyone. I am culturing hIPSC in mTeSR-1 medium. I found some small round white particles persistently attached to cell colonies, and some actively moved under the 40X objective. Also,...
08 June 2024 6,797 5 View
Dear Colleagues, I am currently working on a project that involves differentiating human embryonic stem cells (hESCs) or induced pluripotent stem cells (iPSCs) into mesenchymal stem cells (MSCs)....
19 May 2024 3,164 2 View
Maintaining a WiCell fibroblast-derived line of iPSC in monolayer with laminin coating and E8 media, then using Wnti directed protocol (Gsk3i day 0, Wnti with conditioned media day 3, insulin day...
02 May 2024 6,314 1 View
Hello everyone, I work with skin cells and am performing RT-qPCR of primary fibroblast cells and primary keratinocyte cells to further use as a comparison to iPSC-derived skin cells and 3D skin...
22 April 2024 6,181 2 View
Hi all, In my lab we are currently differentiating neurons from hiPSCs according to the protocol used in Satir, T et al, 2020 (doi: 10.1038/s41598-020-57516-7). We have been successful in the...
27 March 2024 7,900 0 View
Hello everyone, in our team we have established the use of NGN2 to differentiate hiPSC into neurons. We do so by transducing neurons with two lentiviruses, one with inducible NGN2 and puro...
07 March 2024 7,758 6 View