How can I specify the sulfuric acid penetrated into the lattice structure of the gypsum?

I am going to refine the gypsum with sulfuric acid. How do you measure the amount of sulfuric acid that enters the plaster during this process? And I want to find the most effective way to...

04 March 2018 7,065 1 View

Anyone here have luck transfecting AGS suspension cells?

So I have been using Fugene HD for my transfections with AGS and haven't had any major issues except that I can't perform large cell volume transfections due to cost and availability of Promega's...

07 August 2016 4,108 4 View

Are these methods good to see NF-kB activation?

24 May 2016 3,436 2 View

Is there any way to set options of non-linear core correction in VASP or Quantum Espresso?

15 May 2016 2,670 2 View

HiPSC positive for GLAST in FACS - why?

Hey, I'm working on hiPSC differentiation into astrocytes. I stained my cells (hiPSC stage) with a GLAST (ASCA1) antibody for FACS and the cells showed a strong positive signal. As far as I know,...

09 February 2021 3,383 4 View

What is a suitable method to freeze feeders-free, serum free episomal hiPSC?

Hi everyone, I need your professional advice: I started growing episomal iPSC (commercial cell line). I grow them in StemFlex medium. What would you recommend as the most effective freezing...

07 February 2021 8,591 4 View

Are these IPScs Differentiated ?

Hi everyone, So these are images of IPSCs 2 days post passaging/splitting. I noticed that the overall morphology of colonies are not compact or round and without distinct border. However, at...

28 December 2020 3,503 3 View

What really is "Prechordal Plate"?

There is too much discrepency among the textbooks of vertebrate embryology. There are two interpretations of the term "Prechordal plate" in different books: It is the cranial most part of the...

24 November 2020 8,537 1 View

How to stain iPSC derive motor neuron in 96-well plate without detachment?

Hi all, I am using 96-well plates to culture iPSC derived neurons, the problem is, when doing ICC, my neurons tend to detach during the washing procedure, till the final step nearly all neurons...

14 July 2020 3,828 7 View

Why does my H9 IPSC die reasonless after 80% confulency?

Dear All, I am working with thermo fisher H9 cell line, human induced pluripotent stem cell. I made a mutation on them with crispr cas-9 I obtain single colonies and I propagate my clones. I...

13 June 2020 9,046 3 View

Is this be a fungal/bacteria contamination in iPS cell culture?

Dear colleagues, I'm working with iPS cell culture for the first time and after dissociating and placing them in a plate coated with matrigel, they looked like the photo. Could anyone tell me if...

21 December 2019 2,888 14 View

Is this be a fungal/bacteria contamination in iPS cell culture?

Dear colleagues, I'm working with iPS cell culture for the first time and after dissociating and placing them in a plate coated with matrigel, they looked like the photo. Could anyone tell me if...

11 December 2019 3,570 6 View

How to eliminate a single component from a medium?

I'm planning to change the components of cell culture media for reprogramming iPSC in order to make it cost-effective for lab research. some research was made in related areas, but my question is...

10 November 2019 9,457 3 View

Why there is no neutrophil progenitors in human as Hoxb8cells in mice which can produce neutrophils?

As question above is there any certain reason that makes human neutrophil progenitors which can produce neutrophils unavailable? I only know HL60 cells and people differentiate neutrophils from...

10 November 2019 7,763 1 View