Why this HPLC data show negative peak?

Lysine was dissolved in mobile phase and injected in HPLC but there are two peaks. One for positive at 2.1 min and another for negative at 2.8 min? Contents of moilbe phase are ACN:Water:TFA =...

08 July 2020 2,695 13 View

How do I detect sample cross-contamination in a batch of SNP array data?

I will be using Axiom Genome-wide Human Origins 1 array. Is there a way to tell whether a sample is contaminated with DNA from another sample during the extraction stage (for example, excessive...

06 March 2020 7,896 3 View

Where is the proper cooling rate measurement position in the transverse-varestraint test(GTWA)?

Maximum crack length and cooling rate measurements are required to calculate the BTR from the transverse-varestraint test. In previous studies, it is stated that the weld pool should be measured...

13 November 2018 9,486 1 View

How to download/purchase the software Winclada ?

I am looking for a website to download/purchase the program. However, the website cladistics.com is not available now. If someone know the way, please let me know.

06 July 2018 6,097 3 View

What happens if broken target used in magnetron sputter?

I deposit SiO2 thin films using magnetron sputter(RF power) and also found the target broke. Buying a new target is unavailable. The RF power sometimes break down when sputtering the...

29 May 2018 6,233 1 View

How can I specify the sulfuric acid penetrated into the lattice structure of the gypsum?

I am going to refine the gypsum with sulfuric acid. How do you measure the amount of sulfuric acid that enters the plaster during this process? And I want to find the most effective way to...

04 March 2018 7,065 1 View

Is there any possibility of multiple transposon insertions into a chromosome of E. coli?

I learned that a mariner transposon requires only TA dinucleotides for transposition on some DNA strand. (no special sequence prefernce) Let's assume that a cut-and-paste mariner transposon exist...

18 January 2018 8,946 3 View

How can I get clear plant tissue with phloroglucinol staining?

Hi, I am trying to observe lignin through phloroglucinol staining with arabidopsis (petal and sepal). The protocol I am using : 2% phloroglucinol in 100% EtOH , O/N...

31 October 2017 1,975 1 View

SEM preparation for Colepidae (Ciliophora)?

I have a problem for preparing the SEM specimen of Colepidae. The amour plates of the species appears to melt during the preparation procedure. I have attached my preparation and it was prepared...

08 September 2017 6,477 7 View

Do I have to make longer or shorter primer when the PCR is not working

15 August 2017 4,819 24 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Is there any effect of different colours of light (white, red, yellow, blue etc.) on the rate of photosynthesis? If yes, why?

Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....

02 March 2021 7,890 2 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

In an eye with Tyndall, does each dot correspond to an individual cell?

When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...

27 February 2021 6,326 2 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View