8 Questions 13 Answers 0 Followers
Questions related from Leroy Versteeg
We are trying to differentiate THP-1 cells to macrophages and dendritic cells. For macrophages we culture the THP-1’s with 10 ng/mL PMA for 3 days and then we rest them for 3 days more. For...
04 April 2019 4,348 6 View
I am working on a experiment to analyze the immune synapse between T cells and macrophages. In our protocol to stain the cells with fluorescent markers, we have quite a few pipetting, washing and...
09 September 2018 6,694 5 View
Hi all, Recently I have been working on analyzing the immune synapse formation using imaging flow cytometry. I co-culture mouse APCs and T cells and subsequently perform fixation/permeabilization...
04 April 2018 9,619 2 View
Hi! I am testing different vaccine adjuvants and their interaction with RAW 264.7 mouse macrophage cells. I am specifically interested in phagocytosis of the adjuvants. After phagocytosis, cell...
11 November 2017 3,732 3 View
In our lab we have had discussions whether we should represent our flow cyotmetry data as fold-change or background subtracted. We vaccinate animals with antigens and later re-stimulate the...
07 July 2016 8,428 2 View
Hi there! In our lab we shot some cool timelaps videos of parasitic worms under a high-resolution light microscope, showing how the eggs of these worms are moving trough their body and how they...
03 March 2016 9,944 0 View
Hi guys, I am trying to find the best approach to assign a value to samples that do not reach the baseline value in ELISA. In my ELISA assay I perform a 2-fold serial dilution curve starting at a...
01 January 2016 3,105 9 View
I am looking into the plates that are optimal to re-stimulate murine splenocytes in, and subsequently use the same plate to perform ICS in. There are non-tissue culture treated plates...
08 August 2015 8,865 4 View
