I will be running an ELISA and I would like to try using a carbonate-bicarbonate coating buffer with a 9.6pH. Does anyone know the correct amounts or percentages of the substances to dissolve into solution?
Coating Buffer 3.7 g Sodium Bicarbonate (NaHCO3) 0.64 g Sodium Carbonate (Na2CO3) 1 L of distilled water
https://www.bethyl.com/content/protocol_elisa_p3
Most manufacturers recommend 0,05 M carbonate-bicarbonate coating buffer (pH=9,6)
Recipe - 50mM carbonate/bicarbonate buffer:pH 9.6:
• Na2CO3 1.59g (n = 1,59/106=0,015 M )
• NaHCO3 2.93g (n = 2,93/84=0,035 M )
• Dissolve in 1 liter deionized water.
Coating Buffer, 0.05 M Carbonate-Bicarbonate, pH 9.6
3.7 g Sodium Bicarbonate (NaHCO3) 0.64 g Sodium Carbonate (Na2CO3) 1 L of distilled water
- Badges
- Science topic
- Similar topics
- Biological Science
- Kinesiology
- Running