What is the recipe to make a carbonate-bicarbonate coating buffer for ELISA?

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Adam B Shapiro

Coating Buffer 3.7 g Sodium Bicarbonate (NaHCO3) 0.64 g Sodium Carbonate (Na2CO3) 1 L of distilled water

https://www.bethyl.com/content/protocol_elisa_p3

Antonina Kokisheva

Most manufacturers recommend 0,05 M carbonate-bicarbonate coating buffer (pH=9,6)

Recipe - 50mM carbonate/bicarbonate buffer:pH 9.6:

• Na2CO3 1.59g (n = 1,59/106=0,015 M )

• NaHCO3 2.93g (n = 2,93/84=0,035 M )

• Dissolve in 1 liter deionized water.

Jasim Al-saadi

Coating Buffer, 0.05 M Carbonate-Bicarbonate, pH 9.6

3.7 g Sodium Bicarbonate (NaHCO3) 0.64 g Sodium Carbonate (Na2CO3) 1 L of distilled water

Lauren Barbarich

Great, thank you!