Lauren Barbarich

7 Questions 11 Answers 0 Followers

Questions related from Lauren Barbarich

What can be inferred from ELISA OD values that are higher for wells tested with no capture antibody versus wells with capture antibody?

I am trying to develop and optimize my own indirect sandwhich ELISA by performing cherkerboard titration. I am currently checking different dilutions of the capture antibody against the antigen...

08 August 2019 8,238 8 View

How do you analyze ELISA OD values to determine the best concetrations to work with?

I am developing and optimizing an ELISA and I was jsut wondering how you determine what the best concentrations of antibody and antigen are based off the OD values. I have performed checkerboard...

08 August 2019 6,405 4 View

What is the recipe to make a carbonate-bicarbonate coating buffer for ELISA?

I will be running an ELISA and I would like to try using a carbonate-bicarbonate coating buffer with a 9.6pH. Does anyone know the correct amounts or percentages of the substances to dissolve into...

07 July 2019 4,693 4 View

How do you set up a protocol on a plate reader for a checkerboard titration?

I am using a Gen 5 plate reader system and I am trying to set up a protocol to read a checkerboard titration for an indirect sandwich ELISA I am trying to develop. Would it be correct to define...

07 July 2019 1,695 1 View

Any reccomendations for an ELISA microplate washer?

I am looking into getting a plate washer for performing ELISAs to help with consistency and I was wondering if anyone had any reccomendations.

07 July 2019 780 4 View

Where could I purchase lypholized Factor V powder so I can use it to create a standard curve in an ELISA?

I am developing my own ELISA for Factor V in mice, however I am having a hard time figuring out how I will be able to run a standard curve. I know people usually use a lypholized powder at a known...

07 July 2019 346 3 View

What is the correct dilution for a detecting antibody in an ELISA?

I have 0.05 ml of an HRP conjugated antibody that is at a concentration of 1.0 mg/ml. The company reccomends starting at a 1:5,000 dilution, but proper working dilution needs to be optomized....

06 June 2019 2,933 2 View