Why i do get black particles in cell culture and i also have dead cells adhered to my grown live cells and its like just waves?

I grow LC-540 cells and my cells are clear when fully grown image 1, but when I trypsinize the same plate I see black particles image 2. When I subculture them on a new plate I don't see those...

02 March 2020 6,467 1 View

Help me Identify the problem with these cells (LC540 Leydig cells)?

I grow LC-540 Leydig cells, the problem with me is as shown in the picture a. The ones rounded in yellow circles are black particles attached to the plate loosely it does not show any movement,...

03 April 2019 5,164 5 View

Oocyte swelling assay detail protocol. Aquaporin inhibition studies. Any suggestions for other studies?

I am completely unaware of swelling assay as i am from a different back ground. I have a compound which i hypothesize that it will inhibit aquaporin. I am not sure that it would make any gene or...

02 March 2018 2,823 0 View

Does high or low glucose has its effect on total AMPK (not phosphorylated AMPK) in cells during treatment?

I have been using endothelial cells with various conc of glucose from 1.5mM to 30mM. I have changes in total AMPK protein expression using AMPK alpha1/2 santa cruz. I just went through some...

04 May 2016 8,357 2 View

Best compound or drug to inhibit aquaporin expression other than mercury?

I have been working on aquaporins and literaturre says merrcury inhibits AQPs. Few published articles say Thrombin, TPA, bumetadine, Propofol pholoretin etc.. CAn someone suggest me the best among...

02 March 2016 7,310 8 View

This picture is LC540 leydig cells... The red arrows indicate black dots and the yellow arrows indicates bubbles. What are these stuff?

I'm new to cell culture and i have started with Lc540 leydig cells i started growing the cells and i notice these black dots as shown in the picture in red arrows and it doesn't affect my culture...

08 September 2015 3,987 8 View

How do you mimic diabetes conditions in-vitro to study the effect on reproductive organs (e.g. in testes)?

Diabetes has many complications, one of which is on reproduction (infertility). How can I mimic diabetes condition in a testes cell line? I have seen papers in which a pancreas (beta cell line)...

02 March 2014 7,622 7 View

Why are wistar rats specifically used in diabetic models?

I have also seen in many articles using SD rats, but mostly wistar rats are used is there.

01 February 2014 3,313 2 View

Why are my rats dying after 48 hrs when I induce them with STZ 65?

Rats 0f 250-300mg each mg/kg.

01 February 2014 645 14 View

How do you mimic diabetes conditions in LC-540 (lyedig cell)?

I have seen many papers in past using LC-540 grown in high low glucose, but I haven't seen any papers in recent years doing so. I just need to clarify that if i can use high glucose and low...

01 February 2014 8,552 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View