Arun Kannan

13 Questions 22 Answers 0 Followers

Questions related from Arun Kannan

Why i do get black particles in cell culture and i also have dead cells adhered to my grown live cells and its like just waves?

I grow LC-540 cells and my cells are clear when fully grown image 1, but when I trypsinize the same plate I see black particles image 2. When I subculture them on a new plate I don't see those...

03 March 2020 6,601 1 View

Help me Identify the problem with these cells (LC540 Leydig cells)?

I grow LC-540 Leydig cells, the problem with me is as shown in the picture a. The ones rounded in yellow circles are black particles attached to the plate loosely it does not show any movement,...

04 April 2019 5,277 5 View

Oocyte swelling assay detail protocol. Aquaporin inhibition studies. Any suggestions for other studies?

I am completely unaware of swelling assay as i am from a different back ground. I have a compound which i hypothesize that it will inhibit aquaporin. I am not sure that it would make any gene or...

03 March 2018 2,915 0 View

Does high or low glucose has its effect on total AMPK (not phosphorylated AMPK) in cells during treatment?

I have been using endothelial cells with various conc of glucose from 1.5mM to 30mM. I have changes in total AMPK protein expression using AMPK alpha1/2 santa cruz. I just went through some...

05 May 2016 8,453 2 View

Best compound or drug to inhibit aquaporin expression other than mercury?

I have been working on aquaporins and literaturre says merrcury inhibits AQPs. Few published articles say Thrombin, TPA, bumetadine, Propofol pholoretin etc.. CAn someone suggest me the best among...

03 March 2016 7,402 8 View

This picture is LC540 leydig cells... The red arrows indicate black dots and the yellow arrows indicates bubbles. What are these stuff?

I'm new to cell culture and i have started with Lc540 leydig cells i started growing the cells and i notice these black dots as shown in the picture in red arrows and it doesn't affect my culture...

09 September 2015 4,071 8 View

Any one working in the field of reproduction with sertoli cells?

Sertoli cell line

11 November 2014 6,594 1 View

How do you mimic diabetes conditions in-vitro to study the effect on reproductive organs (e.g. in testes)?

Diabetes has many complications, one of which is on reproduction (infertility). How can I mimic diabetes condition in a testes cell line? I have seen papers in which a pancreas (beta cell line)...

03 March 2014 7,767 7 View

How reliable is an analysis of pancreas on stretozotocin induced diabetic rats?

We know that wen rat is given with streptozotocin beta cells are damaged , further research on pancreas shows it holds good?

02 February 2014 7,378 2 View

How do you mimic diabetes conditions in LC-540 (lyedig cell)?

I have seen many papers in past using LC-540 grown in high low glucose, but I haven't seen any papers in recent years doing so. I just need to clarify that if i can use high glucose and low...

02 February 2014 8,648 0 View

Why are wistar rats specifically used in diabetic models?

I have also seen in many articles using SD rats, but mostly wistar rats are used is there.

02 February 2014 3,402 2 View

Why are my rats dying after 48 hrs when I induce them with STZ 65?

Rats 0f 250-300mg each mg/kg.

02 February 2014 735 14 View

Does PCR once standardized using rat liver differ for another animal? And if yes why is that so?

I have just received rat specific primers, and I have optimized it to my lab conditions and sequenced it fine. My question now I have six rats in each group so will the PCR results in the sense...

02 February 2014 7,885 2 View