Diabetes has many complications, one of which is on reproduction (infertility). How can I mimic diabetes condition in a testes cell line? I have seen papers in which a pancreas (beta cell line) has been grown in high and low glucose to mimic diabetic conditions.
Arun
A widespread approach is the use of hyperglycemia in vitro. Most groups use 5 mM glucose for control and 25 mM for hyperglycemia, mostly for 48 h or more. Please make sure to correct for the elevated osmolality by adding mannitose to the control culture. Good luck! JW
Johannes Waltenberger
Thank you so much for your reply. I pretty much accept your comments, but does the same applies to leydig cells (LC540). It would be more helpful if you could send the reference available. Are these cells capable of taking high glucose as that of pancreas.
I use 25mM to mimick the diabetic conditions with porcine endothelial cells. I never checked the osmolarity will it effect parameters significantly..such as protein and gene expression.
Chaitanya
Yup i agree with you. But, how do u rule out that the effect that has occurred is only due to 25mM effect and not endoplasmic or osmotic stress. Suggest me
I think is the best way for you to make coculture with erythrocytes of diabetic patients with high HbA1c.
Binnur Tuzun do u mean to say culture leydig cells with erythrocytes of diabetic patients
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