Tools to predict protein protein interaction?

Hi, I have a list of some genes and i want to check their interaction with my target. Anyone can suggest some bioinformatic tools that can predict the interaction?. many thanks

08 September 2019 8,727 9 View

Can i add two or more antibodies to one sample for WB?

I am doing experiment on SH-sy5y cells treated with drugs but i am using different antibodies of chromatin. my question is whether i can add these Ab to one sample or i have to do one by one with...

07 August 2018 9,354 4 View

Best Concentration of MPP+ for Histone modification?

Can any one have experience with the use of MPP+ treatment in SH-SY5Y cell lines to study epigenetic modification. I want to to induce Parkinson in cultured cells by treatment with MPP+ but first...

06 July 2018 5,087 2 View

How much volume i need from primer 10uM to get 37 ng/ul concentration?

Hi every body. I have a primer concentration of 10uM and i want to use 37.5 ng/ul of this primer as a template. So how much volume i need to have 37.5 ng/ul concentration. I am dealing with...

10 November 2016 2,249 3 View

Choosing Esp31 Restriction enzyme?

Hi, I am carrying molecular cloning using classical methods. I have check enzymes which provide an understandable pattern so any one tell me if i list features of Esp31 enzyme so what will be the...

10 November 2016 2,597 3 View

Touchdown PCR for primers?

Hi, I am using both pairs gRNAs as a insertion part in the vector. So i have to do touchdown pcr for these pairs to annealed them... so what you will suggest for touchdown pcr steps and cycle...

10 November 2016 9,865 3 View

How to ng concentration of primer?

Hi , I have concentration of primer of 10uM and i want to use 37 ng/ul for my reaction so how much volume i need to add to my reaction from 1ouM. Thank you in advance

10 November 2016 8,299 3 View

Any data on Cerebral Palsy genetics with reference to Pakistan?

For my Thesis i need data or review articles in which genetics and epidemiology is present with reference to Pakistan.

01 February 2016 620 2 View

A recent Review article on "Genetics of Cerebral Palsy" ?

Hello Every Body, Any body can provide a review article on genetics of cerebral palsy with reference to x-linked, autosomal dominat inferitence pattern.

01 February 2016 6,391 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View