Arif Mahmood

10 Questions 16 Answers 0 Followers

Questions related from Arif Mahmood

Tools to predict protein protein interaction?

Hi, I have a list of some genes and i want to check their interaction with my target. Anyone can suggest some bioinformatic tools that can predict the interaction?. many thanks

09 September 2019 8,788 9 View

Can i add two or more antibodies to one sample for WB?

I am doing experiment on SH-sy5y cells treated with drugs but i am using different antibodies of chromatin. my question is whether i can add these Ab to one sample or i have to do one by one with...

08 August 2018 9,403 4 View

Best Concentration of MPP+ for Histone modification?

Can any one have experience with the use of MPP+ treatment in SH-SY5Y cell lines to study epigenetic modification. I want to to induce Parkinson in cultured cells by treatment with MPP+ but first...

07 July 2018 5,132 2 View

Any one can guide me how to rescue PINK1 gene phenotypes using guts microbes?

Hi dear All, I am new to the research area of Bioengineering and I want to design microbes, metabolites or miRNAs which can rescue a gene phenotypes which express in brain. There is many methods...

05 May 2018 2,892 0 View

How much volume i need from primer 10uM to get 37 ng/ul concentration?

Hi every body. I have a primer concentration of 10uM and i want to use 37.5 ng/ul of this primer as a template. So how much volume i need to have 37.5 ng/ul concentration. I am dealing with...

11 November 2016 2,367 3 View

How to ng concentration of primer?

Hi , I have concentration of primer of 10uM and i want to use 37 ng/ul for my reaction so how much volume i need to add to my reaction from 1ouM. Thank you in advance

11 November 2016 8,376 3 View

Touchdown PCR for primers?

Hi, I am using both pairs gRNAs as a insertion part in the vector. So i have to do touchdown pcr for these pairs to annealed them... so what you will suggest for touchdown pcr steps and cycle...

11 November 2016 9,913 3 View

Choosing Esp31 Restriction enzyme?

Hi, I am carrying molecular cloning using classical methods. I have check enzymes which provide an understandable pattern so any one tell me if i list features of Esp31 enzyme so what will be the...

11 November 2016 2,676 3 View

A recent Review article on "Genetics of Cerebral Palsy" ?

Hello Every Body, Any body can provide a review article on genetics of cerebral palsy with reference to x-linked, autosomal dominat inferitence pattern.

02 February 2016 6,442 0 View

Any data on Cerebral Palsy genetics with reference to Pakistan?

For my Thesis i need data or review articles in which genetics and epidemiology is present with reference to Pakistan.

02 February 2016 668 2 View