Any data on Cerebral Palsy genetics with reference to Pakistan?

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Tools to predict protein protein interaction?

Hi, I have a list of some genes and i want to check their interaction with my target. Anyone can suggest some bioinformatic tools that can predict the interaction?. many thanks

08 September 2019 8,727 9 View

Can i add two or more antibodies to one sample for WB?

I am doing experiment on SH-sy5y cells treated with drugs but i am using different antibodies of chromatin. my question is whether i can add these Ab to one sample or i have to do one by one with...

07 August 2018 9,354 4 View

Best Concentration of MPP+ for Histone modification?

Can any one have experience with the use of MPP+ treatment in SH-SY5Y cell lines to study epigenetic modification. I want to to induce Parkinson in cultured cells by treatment with MPP+ but first...

06 July 2018 5,087 2 View

Any one can guide me how to rescue PINK1 gene phenotypes using guts microbes?

Hi dear All, I am new to the research area of Bioengineering and I want to design microbes, metabolites or miRNAs which can rescue a gene phenotypes which express in brain. There is many methods...

04 May 2018 2,847 0 View

How much volume i need from primer 10uM to get 37 ng/ul concentration?

Hi every body. I have a primer concentration of 10uM and i want to use 37.5 ng/ul of this primer as a template. So how much volume i need to have 37.5 ng/ul concentration. I am dealing with...

10 November 2016 2,249 3 View

Choosing Esp31 Restriction enzyme?

Hi, I am carrying molecular cloning using classical methods. I have check enzymes which provide an understandable pattern so any one tell me if i list features of Esp31 enzyme so what will be the...

10 November 2016 2,597 3 View

Touchdown PCR for primers?

Hi, I am using both pairs gRNAs as a insertion part in the vector. So i have to do touchdown pcr for these pairs to annealed them... so what you will suggest for touchdown pcr steps and cycle...

10 November 2016 9,865 3 View

How to ng concentration of primer?

Hi , I have concentration of primer of 10uM and i want to use 37 ng/ul for my reaction so how much volume i need to add to my reaction from 1ouM. Thank you in advance

10 November 2016 8,299 3 View

A recent Review article on "Genetics of Cerebral Palsy" ?

Hello Every Body, Any body can provide a review article on genetics of cerebral palsy with reference to x-linked, autosomal dominat inferitence pattern.

01 February 2016 6,391 0 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Choice of Ab for Co-IP: Does an anti-(tag) antibody (such as anti-flag/ anti-myc) a must if I am using co-transfected cells for Co-IP?

Hi guys, I am trying to detect the binding of 2 proteins by Co-immunoprecipitation. However, the choice of antibody is confusing me. I tried probing the endogenous protein right away but the...

16 February 2021 9,050 5 View

Is Genetic Algorithm usage for Motor Conception FEA optimization a good choice?

Hi RG community, Like the title shows ,i was wondering if choosing the GA for this kind of Optimizaiton is a good choice? For example we have a population of 100 (for 7-8 Optimization Variables),...

14 February 2021 1,160 7 View

What can explain a low expected heterozygosity rate for T. monococcum wild samples (0.09) ?

I conducted an SDS PAGE electrophoresis on wheat wild varieties to separate their glutenins and measure the genetic diversity of my sample. I only ran one electrophoresis. Can a genetic drift...

14 February 2021 3,971 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Can any scholar assist me with an r code for calculating average bias for each parameter of a continuous distribution via simulation?

I want to perform a simulation study to examine the consistency property of parameters of a new distribution through the quantile function. The simulation study will contain many replicates (say...

10 February 2021 180 3 View