Tools to predict protein protein interaction?

Hi, I have a list of some genes and i want to check their interaction with my target. Anyone can suggest some bioinformatic tools that can predict the interaction?. many thanks

08 September 2019 8,727 9 View

Can i add two or more antibodies to one sample for WB?

I am doing experiment on SH-sy5y cells treated with drugs but i am using different antibodies of chromatin. my question is whether i can add these Ab to one sample or i have to do one by one with...

07 August 2018 9,354 4 View

Best Concentration of MPP+ for Histone modification?

Can any one have experience with the use of MPP+ treatment in SH-SY5Y cell lines to study epigenetic modification. I want to to induce Parkinson in cultured cells by treatment with MPP+ but first...

06 July 2018 5,087 2 View

Any one can guide me how to rescue PINK1 gene phenotypes using guts microbes?

Hi dear All, I am new to the research area of Bioengineering and I want to design microbes, metabolites or miRNAs which can rescue a gene phenotypes which express in brain. There is many methods...

04 May 2018 2,847 0 View

How much volume i need from primer 10uM to get 37 ng/ul concentration?

Hi every body. I have a primer concentration of 10uM and i want to use 37.5 ng/ul of this primer as a template. So how much volume i need to have 37.5 ng/ul concentration. I am dealing with...

10 November 2016 2,249 3 View

Touchdown PCR for primers?

Hi, I am using both pairs gRNAs as a insertion part in the vector. So i have to do touchdown pcr for these pairs to annealed them... so what you will suggest for touchdown pcr steps and cycle...

10 November 2016 9,865 3 View

How to ng concentration of primer?

Hi , I have concentration of primer of 10uM and i want to use 37 ng/ul for my reaction so how much volume i need to add to my reaction from 1ouM. Thank you in advance

10 November 2016 8,299 3 View

Any data on Cerebral Palsy genetics with reference to Pakistan?

For my Thesis i need data or review articles in which genetics and epidemiology is present with reference to Pakistan.

01 February 2016 620 2 View

A recent Review article on "Genetics of Cerebral Palsy" ?

Hello Every Body, Any body can provide a review article on genetics of cerebral palsy with reference to x-linked, autosomal dominat inferitence pattern.

01 February 2016 6,391 0 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

What are the conflicts between EIA and environmental engineers and lawyers?

Environmental Impact Assessment (EIA) does not live up to the expectation of environmental lawyers and environmental engineers. In your own opinion, how can the EIA process therefore be made more...

03 February 2021 1,329 6 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View