Tools to predict protein protein interaction?

Hi, I have a list of some genes and i want to check their interaction with my target. Anyone can suggest some bioinformatic tools that can predict the interaction?. many thanks

08 September 2019 8,727 9 View

Can i add two or more antibodies to one sample for WB?

I am doing experiment on SH-sy5y cells treated with drugs but i am using different antibodies of chromatin. my question is whether i can add these Ab to one sample or i have to do one by one with...

07 August 2018 9,354 4 View

Best Concentration of MPP+ for Histone modification?

Can any one have experience with the use of MPP+ treatment in SH-SY5Y cell lines to study epigenetic modification. I want to to induce Parkinson in cultured cells by treatment with MPP+ but first...

06 July 2018 5,087 2 View

Any one can guide me how to rescue PINK1 gene phenotypes using guts microbes?

Hi dear All, I am new to the research area of Bioengineering and I want to design microbes, metabolites or miRNAs which can rescue a gene phenotypes which express in brain. There is many methods...

04 May 2018 2,847 0 View

How much volume i need from primer 10uM to get 37 ng/ul concentration?

Hi every body. I have a primer concentration of 10uM and i want to use 37.5 ng/ul of this primer as a template. So how much volume i need to have 37.5 ng/ul concentration. I am dealing with...

10 November 2016 2,249 3 View

Choosing Esp31 Restriction enzyme?

Hi, I am carrying molecular cloning using classical methods. I have check enzymes which provide an understandable pattern so any one tell me if i list features of Esp31 enzyme so what will be the...

10 November 2016 2,597 3 View

Touchdown PCR for primers?

Hi, I am using both pairs gRNAs as a insertion part in the vector. So i have to do touchdown pcr for these pairs to annealed them... so what you will suggest for touchdown pcr steps and cycle...

10 November 2016 9,865 3 View

How to ng concentration of primer?

Hi , I have concentration of primer of 10uM and i want to use 37 ng/ul for my reaction so how much volume i need to add to my reaction from 1ouM. Thank you in advance

10 November 2016 8,299 3 View

Any data on Cerebral Palsy genetics with reference to Pakistan?

For my Thesis i need data or review articles in which genetics and epidemiology is present with reference to Pakistan.

01 February 2016 620 2 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

Can't recognize exact phase and PDF card no of Silicon carbide in XRD?

We have deposited silicon carbide on carbon substrate using one PVD technique. Due to amorphous behavior of SiC at low deposition temperature, we performed heat treatment of those films at...

22 February 2021 2,072 3 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

How to do mono formylation of N,N-diethyl-3-methoxybenzamide?

I have attempted the formylation of the above mentioned compound using a very well reported protocol using secondary Butyl Lithium and TMEDA at - 78 degrees Celsius. I have done it quite a few...

08 February 2021 2,793 2 View

Statistical methods for comparing gene expression curves?

Hi all, I have generated two gene expression curves by measuring bioluminescence every 10 mins for 12 hrs. These curves tend to overlap at at some time points, while a difference can be seen at...

02 February 2021 2,613 7 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

Which antimicrobial test will be suitable to determine antimicrobial activity of silver nanoparticles?

There are some journal articles that reported well diffusion method to determine the antimicrobial activity of silver nanoparticles. Meanwhile, some reported disc diffusion method. Which method is...

06 January 2021 7,123 4 View

New knockout bacterial strain: Can't electroporate it?

I made a new bacterial strain with an unmarked deletion using 2 step homologous recombination (with sacB counterselection). The full protocol is given in the link at the bottom. The protocol...

01 January 2021 1,580 4 View