Any expertise with DNA quantification in micro-liter volumes with ThermoScientific mico-Drop Plate ?

Farah Fadwa Ben belgacem @Farah-Ben-Belgacem

07 November 2016 0 6K Report

After reading Abs260 of only 2uL of my DNA samples using this uDropPlate I have calculated the DNA concentration following the protocol's equation :

DNA concentration(ug/ml)= Abs260 X 50(ug/ml) X multiplication factor (10mm/0.5mm)

I found that the resulted concentrations are very high and I am significantly overestimating my DNA concentrations.

What is the right calculation to get DNA concentration in my case?

Badges
Science method

More Farah Fadwa Ben belgacem's questions See All

Why my gel electrophoresis has smearing?

Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...

22 July 2024 9,761 5 View

Hot to do MTT assay data analysis?

I have repeated an MTT assay three times using different batches of the same cell line. Each time, I tested the same treatment (different medium) and seeded the same number of cells. Additionally,...

08 July 2024 1,900 1 View

Hello.. please how can I prepare an emulsion with nile red and nile blue dyes for visualization under fluorescent microscope ?

how can I incorporate the dyes to each phase ? do I have to heat them !!

21 June 2024 9,377 1 View

I need content writers, poets, researchers to my new online magazine, would you be interested?

Good day! I am the CEO of an online magazine called Order Of The Pen . We specialize in promoting new writers via our platform on Wix and Partnership with Mailchimp for email marketing,...

18 June 2024 4,117 0 View

3T3-F442A cells not adhering to coverslip?

Hi, I am attempting to culture 3T3-F442A cells and carry out IF staining. I'm testing a variety of different compounds on the cells (e.g. lopinavir, clozapine, olanzapine etc.) Up until recently,...

16 June 2024 2,754 0 View

Is the Journal of Pedagogical Research is a trusted journal to publish in? I check in scopus and it is there but I have concerns if it is predator?

Is the Journal of Pedagogical Research a trusted journal to publish in? I checked in scopus website and scimago and I find it is there with scopus Q2 rank; but I have concerns if it is predator?...

08 June 2024 7,678 2 View

How to plot in Simu5G on Omnet++?

Dear network I need to plot instantaneous and mean End-to-end delay in D2D and eNode-based topologies? How to generate/visualise the accurate vec file data? I appreciate your help

14 May 2024 2,897 1 View

What is the best control for conditioned medium?

I'm planning a small research project involving conditioned medium from cancer cells on a human cell line (specifically KGN cells). I intend to expose the cancer cells to this medium for 72 hours...

06 May 2024 8,470 2 View

Topics for my PhD in TOPSIS?

I'm considering research topics for my PhD in TOPSIS methodology. Could you suggest some potential titles or areas of study that would be both relevant and impactful?

26 April 2024 602 2 View

Is this journal legitimate (Tobacco Regulatory Science ISSN: 2333-9748 ) ?

I'm considering submitting a manuscript to this journal (Tobacco Regulatory Science ISSN: 2333-9748 ) Has anyone published in this journal before? I am looking to confirm that this journal is...

05 March 2024 4,144 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View