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Questions related from Farah Fadwa Ben belgacem
First, how to isolate DNA from effective microorganisms? is it the more to genomic DNA isolation or metagenomic DNA isolation protocol? Second, is the Bacterial 16s rRNA Amplicon Sequencing with...
24 September 2019 8,538 8 View
For my recombinant enzymes, I am not able to see any peak in the elution side of the FPLC results using his-tag column, while a peak is present before elution. is anyone able to interpret what...
20 August 2018 7,119 2 View
I have faced this with more than one gene. in the step of gene annotation of my NGS data I found in many case my enzyme of interest but I found that this enzyme is found in multispecies, it means...
09 December 2017 2,752 1 View
what ca we do if we are sure that one of 100 of bacteria has desired gene (which we have its DNA sequence) and we want to know which one has it? to explain more: I have sequenced 100 fosmid with...
27 November 2017 6,017 4 View
With the aim to find special genomic functions we have sequenced metagenomic DNA using Illumina Hiseq. As this method gives the raw data in FastQ format we need to verify the quality before...
31 March 2017 5,206 5 View
I am planning to amplify a large DNA sequence of 40kb with PCR. Can I use a normal Taq Polymerase? Is there any specific one for large sequences amplification?
05 December 2016 3,896 29 View
I have run recombinant Fosmids DNA (around 50kb) on agarose gel electrophoresis, I could visualize only smears and I have remarked that the wells contain DNA as well so may be my fosmids did not...
07 November 2016 4,103 6 View
Dear all We have prepared metagenomic DNA library and now we are screening this library following the high throughput screening method and using fluorogenic substrates to find Hydrolytic enzymes....
16 February 2016 7,157 2 View
Dear all Can any one suggest some statistical software used to analyse screens of high throughput screening and hopefully it is applicable for our case (looking for hydrolytic enzymes using...
16 February 2016 2,602 3 View
I am about to perform metagenomic DNA extraction, and one of the extraction buffer composition is CTAB (Cetyl Trimethyl Ammonium Bromide), unfortunately we don't have CTAB here.
14 January 2016 6,343 2 View
Potassium acetate is a good buffer used in high throughput screening for cellulose-degrading enzymes, can we replace it with sodium acetate or other buffer?
14 January 2016 6,075 5 View
In High throughput screening for cellulase using fluorogenic substrate, how can we ensure that we still can see activity from enzymes that have higher Km values? (accept increase molarity of the...
06 January 2016 6,038 4 View
I am doing High Throughput Screening HTS of functional metagenomic library to screen for cellulose-degrading enzymes using fluorogenic substrate, I want to know what is the best lysis solution for...
05 January 2016 10,009 3 View
