I'm planning a small research project involving conditioned medium from cancer cells on a human cell line (specifically KGN cells). I intend to expose the cancer cells to this medium for 72 hours and then use it on the KGN cells. However, I'm concerned about finding the best control condition. We know that cells consume nutrients from the medium, and using conditioned medium versus fresh medium might not be optimal.
I would greatly appreciate hearing your opinions and suggestions on this matter.
Incubate the culture medium in cell-free dishes or flasks. This will allow for heat, evapoation and culture substatum contact changes that would occur in the conditioned medium. Dilute with fresh medium in the same ratio as your conditioned medium.
Hello Ron Ben David
I agree with Prof. Ellen A G Chernoff .
You should incubate the culture medium alone without cells say for 24 hours. Then use this medium as the control. For instance, if you are using 20% conditioned medium + 80% culture medium, then your control will be 20% of that medium which has been incubated without cells for 24 hours + 80% culture medium.
Best.
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