I will have start working with DAOY cells in the near future and I curious about your experiences with these cells. Are there any unusual specialities in respect to culturing and transfection of these cells?
I'll have to transfect them for Reporter Gene Analysis and found Lipofectamin2000 should work well. Since this is a pretty expensive reagent I was wondering if anyone has successfully used Calcium-Phosphate method or Polyethylenimine for transfection.
Thanks in advance.