Kai F. Albring

5 Questions 129 Answers 0 Followers

Questions related from Kai F. Albring

Any experience with the culture conditions and transfection of DAOY cells?

I will have start working with DAOY cells in the near future and I curious about your experiences with these cells. Are there any unusual specialities in respect to culturing and transfection of...

02 February 2014 4,932 3 View

Can anyone help with identification of an internal ribosomal entry site (IRES-element) in a human mRNA?

I was wondering if anybody has good advice how to analyse the possible presence of an IRES element in a certain mRNA. I recently ran into a novel starting Exon which does not contain any coding...

12 December 2013 1,916 2 View

How does your lab validate the correct identity of a cell line?

Did it ever happen to you, that you thawed a cell line frozen in the N2-tank years ago by some former lab member and you start wondering if you are really dealing with the cell line you think you...

04 April 2013 3,025 17 View

How does your lab treat colleagues that keep messing up instruments used by the whole Institute?

Today I was just wondering again how other labs treat people that keep on messing up instruments like centrifuge rotors, scales, clean benches for cell cultures and so on. How does your boss...

04 April 2013 9,982 12 View

Protocol for total protein extraction from various mouse or rat tissues

I want to analyse the expression patterns of my protein of interest in various tissues such as brain, liver, intestine, kidney, spleen etc. Does anybody know a good protocol, which is suitable to...

11 November 2012 3,039 23 View