I will have start working with DAOY cells in the near future and I curious about your experiences with these cells. Are there any unusual specialities in respect to culturing and transfection of these cells?
I'll have to transfect them for Reporter Gene Analysis and found Lipofectamin2000 should work well. Since this is a pretty expensive reagent I was wondering if anyone has successfully used Calcium-Phosphate method or Polyethylenimine for transfection.
Thanks in advance.
Dear Kai, for those cells I can recommend DreamFect Gold which is also a lipid based transfection reagent or the other option would be to use Magnetofection which is based on magnetic nanoparticles and for your cells NeuroMag seems to be the most suited since it is very effiicient in transfecting neuronal cells and all kind of primary neurons
Let me know if you need more information or publications showing that these reagents are indeed effective ([email protected]). All the best Olivier
Hi Olivier,
thank you for your reply.
A free sample of DreamFect Gold and NeuroMag would be great.
I tried fugeneHD, Lipofectamine, VIvafect, Metafectene, Calciumphosphate, Turbofect, Selfmade Polyethyleneamine and Viromer RED/YELLOW.
With a Dual-luciferase as a read-out I obtained the highest and most consistent transfection with Viromer RED and Vivafect.
Taking costs into account I have to admit, that selfmade PEI also did a good job regarding consistency of results, but showed lower transfection efficiency in respect to luciferase activity.
Unfortunately I didn't get to test DreamFect Gold or NeuroMag yet.
Best,
Kai
Hi Kai,
I just send you a message to get your contact and shipping information
Best Regards,
Olivier
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