Multiple eukaryotic genes under the same promoters?

16 March 2017 4,419 3 View

Does anyone know a data base where I can catch a list of anaerobic and/or aerobic-tolerant animal organisms?

I want to compare protein sequences of proteins shared between aerobic and anaerobic organisms

20 September 2015 9,640 1 View

TreeFix problem

I am facing a problem with TreeFix and I was wondering if you can help me. I found out your corresponding address into TrreFix web page.When I try to launch TreeFix I receive an error message;...

11 January 2015 870 1 View

Can anyone advise me books dealing with phylogenetic reconstruction, mostly in its practical aspects?

I am interested in learning more in depth this topic whereby I have been already introduced and I have a limited experience.

04 December 2014 3,209 16 View

Does anyone know a protocol for a native PAGE gel?

I have to analyze a protein whose pI=8.8. I need to check the difference in electrophoretic motility with and without a ligand; I was wondering about the high pI, if someone know a good...

31 August 2014 7,112 3 View

Does anyone know of organisms that lack catalase?

I was wondering if there are organisms lacking catalase. I know that Plasmodia and Schistosoma species do not possess this enzyme. Thanks.

26 June 2014 334 8 View

How can I solve my BglII site destruction problem?

Hi all, I need to excise a sequence from a plasmid, but this sequence was cloned in a BglII site with BamHI sequence, destroying the BglII recognition sequence. Unfortunately, the new BamHI is...

23 March 2014 7,195 9 View

Intronic miRNA

I'm quite new in the field, and I'm looking for a software or (better) an online platform to analyze intronic sequences in my gene-of-interest, to discover if there are potential miRNA inside the...

19 January 2013 7,742 3 View

Human Microglia Cell Line

I'm looking for a human microglial cell line, any suggestion? I've read about the HMO6 cell line, produced by Nagai et al., but I cannot find if they are commercially available or not.

15 January 2013 5,268 54 View

Can I store the excised lane from the Blue Native Gel overnight? In which buffer? Is there any risk of protein diffusion?

My problem is that in theory, I have to make the first dimension (native) run and the second dimension (SDS-page) run on the same day, and then transfer on membrane still on the same day. Bu also...

02 December 2012 2,612 4 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View