Multiple eukaryotic genes under the same promoters?

16 March 2017 4,419 3 View

Does anyone know a data base where I can catch a list of anaerobic and/or aerobic-tolerant animal organisms?

I want to compare protein sequences of proteins shared between aerobic and anaerobic organisms

20 September 2015 9,640 1 View

TreeFix problem

I am facing a problem with TreeFix and I was wondering if you can help me. I found out your corresponding address into TrreFix web page.When I try to launch TreeFix I receive an error message;...

11 January 2015 870 1 View

Can anyone advise me books dealing with phylogenetic reconstruction, mostly in its practical aspects?

I am interested in learning more in depth this topic whereby I have been already introduced and I have a limited experience.

04 December 2014 3,209 16 View

Any experience in transfecting Daoy cells?

Hi all, I'm starting to use Daoy cells (medulloblastoma), and I would need to transfect them. In literature seems that there is no info on that...Has anyone tried that? thanks

23 October 2014 5,671 4 View

Does anyone know a protocol for a native PAGE gel?

I have to analyze a protein whose pI=8.8. I need to check the difference in electrophoretic motility with and without a ligand; I was wondering about the high pI, if someone know a good...

31 August 2014 7,112 3 View

Does anyone know of organisms that lack catalase?

I was wondering if there are organisms lacking catalase. I know that Plasmodia and Schistosoma species do not possess this enzyme. Thanks.

26 June 2014 334 8 View

How can I solve my BglII site destruction problem?

Hi all, I need to excise a sequence from a plasmid, but this sequence was cloned in a BglII site with BamHI sequence, destroying the BglII recognition sequence. Unfortunately, the new BamHI is...

23 March 2014 7,195 9 View

Intronic miRNA

I'm quite new in the field, and I'm looking for a software or (better) an online platform to analyze intronic sequences in my gene-of-interest, to discover if there are potential miRNA inside the...

19 January 2013 7,742 3 View

Human Microglia Cell Line

I'm looking for a human microglial cell line, any suggestion? I've read about the HMO6 cell line, produced by Nagai et al., but I cannot find if they are commercially available or not.

15 January 2013 5,268 54 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View