Fulvio Celsi

5 Questions 62 Answers 0 Followers

Questions related from Fulvio Celsi

Any experience in transfecting Daoy cells?

Hi all, I'm starting to use Daoy cells (medulloblastoma), and I would need to transfect them. In literature seems that there is no info on that...Has anyone tried that? thanks

24 October 2014 5,742 4 View

How can I solve my BglII site destruction problem?

Hi all, I need to excise a sequence from a plasmid, but this sequence was cloned in a BglII site with BamHI sequence, destroying the BglII recognition sequence. Unfortunately, the new BamHI is...

24 March 2014 7,269 9 View

Intronic miRNA

I'm quite new in the field, and I'm looking for a software or (better) an online platform to analyze intronic sequences in my gene-of-interest, to discover if there are potential miRNA inside the...

20 January 2013 7,809 3 View

Human Microglia Cell Line

I'm looking for a human microglial cell line, any suggestion? I've read about the HMO6 cell line, produced by Nagai et al., but I cannot find if they are commercially available or not.

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Can I store the excised lane from the Blue Native Gel overnight? In which buffer? Is there any risk of protein diffusion?

My problem is that in theory, I have to make the first dimension (native) run and the second dimension (SDS-page) run on the same day, and then transfer on membrane still on the same day. Bu also...

03 December 2012 2,685 4 View