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Hi all, I'm starting to use Daoy cells (medulloblastoma), and I would need to transfect them. In literature seems that there is no info on that...Has anyone tried that? thanks
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Hi all, I need to excise a sequence from a plasmid, but this sequence was cloned in a BglII site with BamHI sequence, destroying the BglII recognition sequence. Unfortunately, the new BamHI is...
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This may seem a naive question (and indeed it is); I'm in the process of using the dual luciferase (firefly and renilla) to quantitate the activity of my promoter of interest. My question is:...
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I'm in the process of analyzing the results from an Illumina Exome chip. I have only the final_report.txt file (and the HumanExome-12v1-1_A_Gene_Annotation.txt file for matching) and I do not...
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I'm quite new in the field, and I'm looking for a software or (better) an online platform to analyze intronic sequences in my gene-of-interest, to discover if there are potential miRNA inside the...
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I'm looking for a human microglial cell line, any suggestion? I've read about the HMO6 cell line, produced by Nagai et al., but I cannot find if they are commercially available or not.
16 January 2013 5,351 54 View
My problem is that in theory, I have to make the first dimension (native) run and the second dimension (SDS-page) run on the same day, and then transfer on membrane still on the same day. Bu also...
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