Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Have you applied 3D Laser scanning and Building Information Modeling for facility management during operation phase?

14 March 2020 7,806 3 View

Is there any difference between Authoritarian Mother and Authoritarian Father?

Good day! I'm looking for journal articles that would help me differentiate between an authoritarian mother and an authoritarian father. Thank you in advance!

28 February 2018 5,212 3 View

Cost-effectiveness model: Converting different rates on different rates into one probability?

Hello Everyone, Need quick help! I have 5 different rates for recurrence from 5 studies with different followup. How do I combine them to find one probability to be used in cost-effectiveness...

05 November 2017 4,107 7 View

Are there known antibodies produced when a human is infected with STH (Ascaris lumbricoides, Trichuris trichiura, hookworm)?

Are there known antibodies produced when humans are infected with STH (Ascaris lumbricoides, Trichuris trichiura, hookworm) and do the produced antibodies differ per helminth? Thanks in advance!

19 September 2017 1,116 5 View

How long we can keep soil water extraction in refrigerator (4oC) ?

07 August 2017 6,149 6 View

Is there an assay that is able to quantify the degradative enzymatic activity of fungi without isolating the enzyme from the fungi?

18 May 2017 8,694 3 View

What are the implications of neutral theory for interpreting patterns of genetic variation in ecology and evolution?

04 November 2016 7,331 4 View

Any changes in soil dissolved organic matter if we conduct second time extraction by hot/cold water?

06 July 2016 8,229 3 View

Any changes in soil dissolved organic matter if we conduct second time extraction by hot/cold water?

05 July 2016 2,288 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Can Immunogenic BNT162b vaccines protect HUMANS from SARS-CoV-2?

"A safe and effective vaccine against COVID-19 is urgently needed in quantities sufficient to immunise large populations. We report the preclinical development of two BNT162b vaccine candidates,...

11 February 2021 6,628 9 View

How to check if the PMF profile obtained from ABF simulation is correct?

I am doing ABF simulations to calculate ligand binding enegy. The PMF profile that I get using single long ABF simulation is attached as pmf_total.png. When I do this ABF calculation with 10...

10 February 2021 4,170 2 View

Ligands with 3,4,5-trihydroxyphenyl group?

Hi In the design of a bidentate ligand with imine group, is it okay to have a 3,4,5-trihydroxyphenyl group in the structure? In other words, does the 3,4,5-trihydroxyphenyl group compete with...

08 February 2021 4,977 7 View

Review Papers regarding transcriptional regulations & promoter types/classes in Gram positive bacteria?

Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!

08 February 2021 6,909 3 View

Is there any difference between "SEP" as a non standard residue and phohophorylated serine?

I am trying to run a molecular dynamics simulation for a ligand that contains a "SEP" residue. also, I am preparing my files using Charmm-GUI using charmm36 ff, but it says that it will rename my...

31 January 2021 6,321 1 View

How to show a protein is being dimerized in SDS-PAGE gel?

A protein dimerizes through CTD. I have expressed CTD of protein with two different tags. Now, I want to show that these CTD of protein dimerize in a pull-down assay. But my doubt is that if the...

29 January 2021 7,529 4 View