We are applying the standard protocol for rapid expansion of tumor-infiltrating lymphocytes. In this protocol, after two weeks growing in culture, the so-called "young TILs" are expanded in the presence of IL-2 3000 IU/ml + antiCD3 OKT3 antibody 30ng/ml + feeder cells (obtained by mixing irradiate PBMCs from three healthy donors).
We are having some problems when thawing the vials of frozen feeders, so we want to explore any other available source of feeder cells, if anyone knows it.
Many thanks!
Hello Juan A. Marín-Jiménez
Artificial antigen presenting cells (aAPCs) expressing ligands for the T cell receptor and costimulatory molecules can activate and expand T cells, while improving their potency and function.
You may refer to the research article attached below where the investigators make use of engineered K562 cell-based aAPCs as an "off-the-shelf" platform for ex vivo TIL expansion. K562 aAPCs that express CD137L offer the potential to expand antigen-experienced TILs and represent a potential new cell-based platform for the standardization of ex vivo TIL expansion.
Article Engineered artificial antigen presenting cells facilitate di...
Best.
Hello Juan, This might be useful if you need to move from irradiation for your feeders.
Article Mitomycin C as an alternative to irradiation to inhibit the ...
I often label the feeders with a tracker dye so they can be eliminated from subsequent flow cytometric analysis.
regards
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