Juan A. Marín-Jiménez

8 Questions 3 Answers 0 Followers

Questions related from Juan A. Marín-Jiménez

What are the optimal conditions to perform scRNA-seq from digested tumor samples?

We are working with patient-derived fresh tumor samples. We perform mechano-enzymatical digestion (DNAse + Colagenes II, 1h aprox., 37ºC) and send the single cell suspension to the scRNA-seq...

26 September 2023 3,498 0 View

Any alternative to irradiated PBMCs as feeder cells for TIL rapid expansion protocol (REP)?

We are applying the standard protocol for rapid expansion of tumor-infiltrating lymphocytes. In this protocol, after two weeks growing in culture, the so-called "young TILs" are expanded in the...

24 January 2023 5,842 3 View

Any advice on expanding tumor infiltrating lymphocytes from immunocompetent mice?

I am trying to model ex vivo TIL expansion protocol using tumor fragments from C57BL6 bearing ortothopic ID8-p53-/- ovarian tumor. Briefly, we culture fragments on T cell media (RPMI glutamax, 10%...

24 October 2022 7,105 0 View

Any recommendations on IHC-paraffin/IF antibodies to detect NK cells and HLA-I?

I am working with patient-derived tumor samples and I would like to detect CD56/NCAM1+ cells as a marker for NK cells and MHC-I molecules (HLA-ABC) in the same slide. Could you recommend...

04 November 2021 1,037 1 View

What gating strategy do you recommend to define immune infiltration percentage in tumor samples?

I am processing and disgregating patient-derived tumor samples for flow cytometry analysis. I use anti-Human CD45 positive to characterize the immune cells within the tumor but not sure what is...

23 September 2021 487 3 View

How to begin evaluation in ProcartaPlex Analyst 1.0 Software?

I am trying to analyze my raw data from Luminex using ProcartaPlex Analyst software v1.0 from Thermofisher. After define input data (setting blanks, standards and unknowns) and select the used...

26 August 2021 7,421 2 View

How can I establish ovarian cancer cell line culture from patient-derived tumor samples?

I am processing high-grade serous ovarian cancer samples and trying to grow tumor cell culture for subsequent functional assays. Any recommendation on the dissociation process, enzyme mix and/or...

23 March 2021 3,791 3 View

Does anyone have experience with ID8-OVA cell line-derived tumor into C57BL/6 mouse model?

I am trying to establish an ovarian cancer preclinical model through peritoneal injection into C57BL/6 mice. The parental ID8 p53-/- cell line was modified through lentiviral infection in order...

15 March 2021 2,879 1 View