I am trying to knock out a gene in a cell line using CRISPR and I have been reading alot about that. I want to use 2 gRNA (crRNA and tracer RNA and not sgRNA) to do a large deletion in a gene. can you give me some advices about that? are there things that I should do/avoid?
how hard can it be? and how long should it take?
many thanks
Pablo Berrocal
If you want to perform a large deletion in a gene, I suggest using two guide RNAs to produce two different double-strand breaks. In that way, the chances of causing a larger deletion are notably increased.
1 votes
0 thanks
- Badges
- Science method
More Danny Had's questions See All
Similar questions and discussions