In simple terms: to what extent do you believe classicial inversion frameworks provide more "general" estimates than learned frameworks?

This is more of a survey question than a query for precise mathematical detailing. Opinions are welcome!

10 September 2020 6,516 2 View

Is Calcium Sulfonate chemically compatible with "Polyester Polyol" types of Polyurethane elastomers?

I have an application where I need to grease a "polyester Polyol" type of Polyurethane. The application requires excellent adhesion, wear resistance, and water wash-out/spray resistance, with good...

21 July 2019 801 5 View

Aluminum air battery crimping?

Dear all, My name is Danny Gelman co-founder of InEnSto, a company developing Aluminum-air battery technology. We are currently working with your AG3 cell casings in order to implement our...

18 June 2018 8,060 3 View

Anyone can help me find an algorithm in R : "Piecewise Direct Standardization" (PDS) for data transfer between FTIR instruments ?

I'm dealing with data from different infrared spectrometers and I would like to transfer data from a slave instrument to a master instrument, in order to apply a classification model built on the...

26 March 2018 5,949 5 View

Antibodies that fail to ChIP-seq?

Hi, We are developing a new ChIP-seq alternative that we think is less sensitive to antibody quality. Can anyone recommend an antibody, preferably CTCF but others will do as well, that works...

06 November 2017 4,700 3 View

What is the mass density of single carbon fibre?

Dear All, I want to calculate the fibre volume fraction of the composite. one of its parameter is the mass density of its reinforcement, which is carbon fibre. I try to find it on internet but the...

14 March 2017 436 3 View

How to calculate flexural properties of sandwich structure?

13 February 2017 371 8 View

How to interpret large discrepancies between BET and DFT surface area of carbon materials?

I have been performing surface area analysis of various carbon materials using a Gemini VII Micromeretics Surface Area analyzer. Typically, I get a full BET and DFT analysis, and the surface areas...

23 October 2016 132 4 View

Best way to quantify chemiluminescent antibody array results?

I am running the PathScan RTK Antibody Array from Cell Signaling and am trying to find a way to quantify my results. The results come out as dots signifying the presence of a certain protein or...

23 June 2016 4,330 3 View

How can i do multiple static analysis in ANSYS ?

09 April 2016 9,715 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View