Does a parents education level have an impact on their child's education?

Do children follow in the same footsteps as their parents in terms of their education?

12 January 2021 1,375 7 View

Protocol for activating PBMCs using PHA/IL-2?

Hi there, just looking for a simple protocol stimulating 1 x 10^6 PBMCs/ mL in RMPI + 10%FBS using PHA and IL-2? I am looking for CD25, NKp44, c-kit expression and a few other markers for flow...

08 December 2020 2,087 1 View

after dissolving biomass in an ionic liquid and filtering it with denoised water. how may i find out how much of cellulose in my filtrate?

i was wondering if you know of an accurate method for quantifying the amount of cellulose in a filtrate. of biomass that had been dissolved in ionic liquid and then filtered with deionized...

05 November 2020 6,469 2 View

Protocol for fixing mouse livers and fat pads for H&E staining?

Hi there! I need advice on a protocol for mouse tissue staining. I will dissect mouse livers and fat pads and look for liver steatosis and adipocyte perimeter. I was planning on doing this with...

22 November 2019 7,490 6 View

Why do my KGN granulosa cells are shrinking when treated with PMA ?

Hello everyone I need help, I am working with KGN granulosa cell line (DMEM/F12 media, 10% FBS, 1% Pen/Strep) I am treating the cells with PMA at 100nM concentration to activate PKC, but...

29 April 2019 5,057 3 View

Which statistical test(s) should be run with ordinal data?

The research has: 1 IV and 3 DV's. Likert scales used yielding scores of 1-5 on Behavioural Observational Scales (BOS). n=99 with each individual being scored on all 3 DV's. Total BOS=297. 99 BOS...

30 November 2018 8,807 4 View

Which statistical test(s) should be run with what kind of data (nominal, ordinal, etc)?

1 IV and 3 DV's. Likert scales used yielding scores of 1-5 on Behavioural Observational Scales (BOS). n=99 with each individual being scored on all 3 DV's. Total BOS=297. 99 BOS scored for each...

29 November 2018 6,231 4 View

What is the best method of alkaloid extraction from insects?

I'm working on a project for which I need to extract the alkaloids in Lycorma Delicatula (the spotted lanternfly) for HPLC, and I'm having a difficult time finding an adequate protocol for...

14 November 2018 9,004 3 View

Does anyone know any good credible questionnaires measuring social media use?

I am looking for any credible questionnaires that are able to measure a person's social media usage. Thank you.

28 October 2018 2,316 3 View

How can I stain cells in culture supernatant ?

I am doing endothelial cells culture and it seems I have erythrocytes contamination but I want to check that. How can I do it as these cells are only in the supernatant ? Thank you in advance

17 June 2018 1,933 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

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28 February 2021 9,936 2 View