Does a parents education level have an impact on their child's education?

Do children follow in the same footsteps as their parents in terms of their education?

12 January 2021 1,375 7 View

Protocol for activating PBMCs using PHA/IL-2?

Hi there, just looking for a simple protocol stimulating 1 x 10^6 PBMCs/ mL in RMPI + 10%FBS using PHA and IL-2? I am looking for CD25, NKp44, c-kit expression and a few other markers for flow...

08 December 2020 2,087 1 View

Any advice on how to get KO cell lines by santa cruz Crispr-cas9 please?

Hello, I tried several times to obtain two KO cell lines for ERN1 (IRE1 alpha) using a santa cruz crispr cas9 "double nickase". (HNSCC cancer cell lines : PECA-PJ41 and PECA-PJ34). I tried...

07 December 2020 8,141 1 View

after dissolving biomass in an ionic liquid and filtering it with denoised water. how may i find out how much of cellulose in my filtrate?

i was wondering if you know of an accurate method for quantifying the amount of cellulose in a filtrate. of biomass that had been dissolved in ionic liquid and then filtered with deionized...

05 November 2020 6,469 2 View

Protocol for fixing mouse livers and fat pads for H&E staining?

Hi there! I need advice on a protocol for mouse tissue staining. I will dissect mouse livers and fat pads and look for liver steatosis and adipocyte perimeter. I was planning on doing this with...

22 November 2019 7,490 6 View

Why do my KGN granulosa cells are shrinking when treated with PMA ?

Hello everyone I need help, I am working with KGN granulosa cell line (DMEM/F12 media, 10% FBS, 1% Pen/Strep) I am treating the cells with PMA at 100nM concentration to activate PKC, but...

29 April 2019 5,057 3 View

Which statistical test(s) should be run with ordinal data?

The research has: 1 IV and 3 DV's. Likert scales used yielding scores of 1-5 on Behavioural Observational Scales (BOS). n=99 with each individual being scored on all 3 DV's. Total BOS=297. 99 BOS...

30 November 2018 8,807 4 View

Which statistical test(s) should be run with what kind of data (nominal, ordinal, etc)?

1 IV and 3 DV's. Likert scales used yielding scores of 1-5 on Behavioural Observational Scales (BOS). n=99 with each individual being scored on all 3 DV's. Total BOS=297. 99 BOS scored for each...

29 November 2018 6,231 4 View

What is the best method of alkaloid extraction from insects?

I'm working on a project for which I need to extract the alkaloids in Lycorma Delicatula (the spotted lanternfly) for HPLC, and I'm having a difficult time finding an adequate protocol for...

14 November 2018 9,004 3 View

Does anyone know any good credible questionnaires measuring social media use?

I am looking for any credible questionnaires that are able to measure a person's social media usage. Thank you.

28 October 2018 2,316 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Is it possible, for a limited dateset of images, to use Convolution filters + Random Forest instead of UNet?

UNet is 'relatively' recent and was invented mainly to deal with biomedical images, yet is it also possible, for a limited dateset of images, to use Convolution filters + Random Forest instead of...

21 February 2021 4,858 3 View

Can I consider the OOB score of RF model as the same as R^2 score?

In a topology optimization machine learning prediction algorithm, can I consider the OOB score of RF as the same as R^2 score. Could that be a more unbiased way of validating the model

14 February 2021 3,540 1 View

Are there any papers that deal with the effects of climate change on the pollination in Tropical orchids?

There are a few papers dealing with the effects of climate change on the pollination of European orchids, but I wonder if similar studies have been carried out in the Tropics.

04 February 2021 1,818 4 View

The Akaike information criterion of the random forest model ?

Please, how to calculate the number of model parameters of the random forest model to determine the Akaike Information Criterion (AIC) using this following expression: AIC = n * log(MSE) + 2 *...

27 January 2021 816 6 View

What is the magnitude of yearly rhizopdeposition in tropical forests?

Hi all, I am trying to find reference values on annual soil rhizodeposition rates for different kinds of forests, with a particular interest for tropical rainforests. Do we have an idea of the...

26 January 2021 4,278 4 View

How can I make unsupervised clustering of trees species using algorithms like HAC, Kmeans... in R?

I am working on the development of multi-species volume allometric equation from diameter (DBH). I would like to form groups of species with the largest possible volume-diameter correlations by...

19 January 2021 5,752 5 View

How to calculate random forest manually?

17 January 2021 301 2 View

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13 January 2021 2,487 1 View

Why do I have good performances for the test set but bad performances in predicting new data ?

Hello :) I'am trying to predict churn for the year of 2020 based on historical data from 2014 to 2019 on R Studio. The aim is to predict churn probability for every person. At the beginning...

13 January 2021 5,565 7 View