Hello all,
I'm looking to measure Ag presentation to T cells on a B cell line HLA. I was hoping that I would be able to conjugate my Ag to a protein tag (FLAG, GFP, etc) and get a functional T cell read out as a proxy for presentation of my Ag. But as far as I can tell there isn't a T cell line with a TCR that is specific for anything like that.
Does anyone have any thoughts or know of any cell lines? I'm working with HIV so an Ag specific T cell line isn't really possible.
Thanks!
To measure B cell antigen presentation, a commonly used specific T cell line is the T-cell hybridoma or T-cell clone that expresses a T-cell receptor (TCR) specific for the antigen of interest.
Read my research about hassle corpus cell and how do cell determine at what size to grow and correlate them
One such line is the T cell hybridoma line B3Z. This line is specific for the chicken ovalbumin peptide SIINFEKL presented by H-2Kb.
Another line is the DO11.10 T cell hybridoma which is specific for the ovalbumin peptide OVA323-339 presented by I-A[b].
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