Isolated yeast genomic DNA (two samples), run on the 0.9%TAE agarose gel. Now could see both samples smear but different migration rate? Is it because of some left over ETOH if happened so while drying up sample? Should I proceed with PCR?
Hello Diven, have you test the A260/280? The ideal number for A260/280 is 1.8-2.0. I think that if A260/280 is lower than 1.8 and with a peak before 260nm maybe due to the ETOH. You may wash the sample again, if you want to do the PCR, I think that the purity is important. Or you want to know whether isolate the genomic DNA successfully, you could use endonuclease, you may get some brands with different size. I hope this will helpful too you. Good luck.
Hi there,
For what purpose do you need this genomic DNA? If it is just for using it as DNA template for PCR you don't need a high purity level. Here the protocol I use for PCR screening on yeast:
http://openwetware.org/wiki/Silver:_Colony_PCR
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